We express the product's activity in TIU (Trypsin Inhibitor Units). Multiply the activity on the Certificate of Analysis by 1300 to convert it to KIU (Kallikrein Inhibitor Units).
A6103
Aprotinin
recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, powder, ≥98% (SDS-PAGE), suitable for inhibition assay
別名:
BPTI, Basic pancreatic trypsin inhibitor, Kallikrein-trypsin inactivator, Kunitz protease inhibitor
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
1 mg | カートの在庫状況を確認する | ￥27,500 |
25 mg | カートの在庫状況を確認する | ￥77,700 |
100 mg | カートの在庫状況を確認する | ￥264,000 |
この商品について
UNSPSC Code:
12352202
NACRES:
NA.77
EC Number:
232-994-9
製品名
Aprotinin Nicotiana tobacco, >= 5TIU/mg protein, >= 98 % SDS-PAGE | 9087-70-1, recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, ≥98% (SDS-PAGE)
recombinant
expressed in Nicotiana (tobacco)
Quality Segment
description
recombinant form of thenative bovine-sequence aprotinin purified from plant tissue (tobacco)
product line
BioUltra
assay
≥98% (SDS-PAGE)
form
powder
specific activity
≥5 TIU/mg protein
mol wt
~_6.5 kDa
technique(s)
inhibition assay: suitable
solubility
0.85% sodium chloride: 5 mg/mL
UniProt accession no.
storage temp.
2-8°C
Gene Information
cow ... PTI(404172)
Preparation Note
Contains no animal-derived components or impurities, and is manufactured by transient expression of the aprotinin message in RNA (+)-strand tobacco mosaic virus vectors propagated in non-transgenic Nicotiana plants. This is a recombinant form of the native, bovine-sequence aprotinin, which is traditionally isolated from bovine lung by methods involving fractional precipitation, gel filtration, and ion exchange chromatography. Unlike animal-derived aprotinin, this product is isolated and purified from plant tissue by proprietary methods.
Other Notes
One trypsin inhibitor unit (TIU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) per min at pH 7.8 at 25 °C.
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当該品目 | |||
|---|---|---|---|
| assay ≥98% (SDS-PAGE) | assay ≥98% (SDS-PAGE) | assay - | assay - |
| Gene Information cow ... PTI(404172) | Gene Information cow ... PTI(404172) | Gene Information cow ... PTI(404172) | Gene Information cow ... PTI(404172) |
| Quality Level 300 | Quality Level 300 | Quality Level 300 | Quality Level 300 |
| form powder | form powder | form lyophilized powder | form lyophilized powder |
| solubility 0.85% sodium chloride: 5 mg/mL | solubility H2O: 5 mg/mL, clear to very slightly hazy | solubility H2O: ≥5 mg/mL | solubility H2O: >10 mg/mL |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
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保管分類
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
A6103-25MG: + A6103-VAR: + A6103-1MG: + A6103-BULK: + A6103-100MG:
jan
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Do you assay this the activity of Product A6103, Aprotinin bovine, in TIU or in KIU?
1 回答-
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At what concentration should I use Product A6103, Aprotinin bovine?
1 回答-
For most applications, 10 to 800 nM works well (based on a molecular weight of 6512 g/mol). This information may be found in the technical bulletin for the Protease Inhibitor Panel, Product No. INHIB1.
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How does Product A6103, Aprotinin bovine, compare to Trasylol?
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Trasylol is the Bayer AG registered trademark name for this material.
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How does the storage temperature relate to shipping conditions?
1 回答-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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How should I store solutions of Product A6103, Aprotinin bovine?
1 回答-
We generally advise storing stock solutions of approximately 5-10 mg/mL. Working solutions down to 0.065 micrograms/mL can be stored refrigerated at 2-8°C for one week, or at - 20°C for appoximately six months in single use aliquots. See Table 2 in the Datasheet for details.
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Product A6103, recombinant bovine aprotinin has a specification of ~60% protein. What is the remaining components of the solid?
1 回答-
Product A6103, recombinant bovine aprotinin is >98% purity by SDS-PAGE. The product is ~60% protein with the balance being salts (NaCl).
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