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Merck

A4187

Sigma-Aldrich

抗ヤギIgG (全分子)-アルカリフォスファターゼ ウサギ宿主抗体

affinity isolated antibody, buffered aqueous glycerol solution

別名:

Rabbit Anti-Goat IgG (whole molecule)–AP

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About This Item

MDL番号:
UNSPSCコード:
12352203
NACRES:
NA.46
現在、価格および在庫状況を閲覧できません。

由来生物

rabbit

品質水準

結合体

alkaline phosphatase conjugate

抗体製品の状態

affinity isolated antibody

抗体製品タイプ

secondary antibodies

クローン

polyclonal

フォーム

buffered aqueous glycerol solution

交差性

goat

テクニック

direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

輸送温度

wet ice

保管温度

2-8°C

ターゲットの翻訳後修飾

unmodified

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関連するカテゴリー

詳細

IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus they play a crucial role in facilitating cytological immune responses. Polyclonal anti-goat IgG (whole molecule)–alkaline phosphatase antibody (diluted 1:15,000) can be used as a secondary antibody for immunoblotting of Sall4. This antibody can also be used in immunohistochemistry (diluted1:400) . Rabbit anti-goat IgG antibody reacts specifically with goat IgG and normal goat serum.
Primary goat antibodies are often used to study target proteins for various clinical and research purposes. Thus, secondary anti-goat antibody conjugated to a detectable substrate can be used to facilitate the accurate detection and localization of target proteins.

特異性

全てのヤギイムノグロブリンに結合します。

免疫原

Purified goat IgG.

アプリケーション

Anti-Goat IgG (whole molecule)-Alkaline Phosphatase antibody is suitable for use in immunoblotting. The product can also be used for direct ELISA (1:30,000) and immunohistochemistry (1:50 using formalin-fixed, paraffin-embedded sections).
Detection of TLR2 and TLR4 in protein extracts from epidermal keratinocytes was performed by western blot using alkaline phosphatase conjugated rabbit anti-goat IgG as the secondary at a 1:2500 dilution.

物理的形状

0.05M Trisバッファ-溶液 (pH 8.0, 1mM MgCl2, 10mM グリシン, 1%BSA, 50%グリセロ-ル, 15mM アジ化ナトリウム含有)

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

10 - Combustible liquids

WGK

WGK 2


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

A4187-.05ML:
A4187-VAR:
A4187-BULK:
A4187-.5ML:
A4187-.25ML:
A4187-1ML:


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Menhaj et al.
Planta, 209(4), 406-413 (1999-11-07)
An antibody was raised against the protein HL#2 which is a nuclear-encoded light-stress-induced protein of barley (Hordeum vulgare L.). The expression of the mRNA and the protein of HL#2 was determined under the influence of high light and methyl jasmonate.
Andor Pivarcsi et al.
International immunology, 15(6), 721-730 (2003-05-17)
Keratinocytes have the ability to kill pathogenic fungi and bacteria by producing antimicrobial substances. Recent studies suggest that microbial components use signaling molecules of the human Toll-like receptor (TLR) family to transduce signals in various cells. Here we provide evidence
Minoru Omi et al.
The Journal of comparative neurology, 519(13), 2615-2621 (2011-04-15)
The optic tectum is a visual center of nonmammalian vertebrates that receives retinal fibers in a retinotopic manner. It has been accepted that retinal fibers project to some superficial laminae of the tectum, but do not go through lamina g
Raquel Alvarado et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(1), 85-95 (2016-09-30)
The NLRP3 inflammasome is a multimeric protein complex that controls the production of IL-1β, a cytokine that influences the development of both innate and adaptive immune responses. Helminth parasites secrete molecules that interact with innate immune cells, modulating their activity
Jesse L Cox et al.
PloS one, 5(11), e15486-e15486 (2010-11-26)
Master regulators, such as Sox2, Oct4 and Nanog, control complex gene networks necessary for the self-renewal and pluripotency of embryonic stem cells (ESC). These master regulators associate with co-activators and co-repressors to precisely control their gene targets. Recent studies using

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