This product has a shelf life of 3 years.
D5637
3,3′-ジアミノベンジジン 四塩酸塩 水和物
≥96%
別名:
(1,1′-Biphenyl)-3,3′,4,4′-tetramine tetrahydrochloride dihydrate
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1 G
￥8,930
5 G
￥29,700
10 G
￥53,300
25 G
￥109,000
50 G
￥165,000
About This Item
化学式:
C12H14N4 · 4HCl · xH2O
CAS番号:
EC Number:
MDL番号:
UNSPSCコード:
12171500
PubChem Substance ID:
NACRES:
NA.47
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品質水準
アッセイ
≥96%
フォーム
powder, crystals or chunks
テクニック
titration: 96-104% (with NaOH)
色
white to light brown, and Faint Red and Faint Grey to Light Grey
mp
280 °C
溶解性
H2O: 50 mg/mL
アプリケーション
diagnostic assay manufacturing
hematology
histology
保管温度
−20°C
SMILES記法
O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
InChI
1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2
InChI Key
DXWSCXIZIHILNP-UHFFFAOYSA-N
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詳細
3,3′-ジアミノベンジジン四塩酸塩水和物（DAB HCl）は、生物学的サンプル中の抗原および抗体の存在を可視化するための免疫組織染色などの染色法に一般的に使用されている色素原です。DAB HClは、細胞中のペルオキシダーゼ活性を可視化するための免疫組織染色として設計されています。DAB酸化は、分光光度計で測定できます。
アプリケーション
3,3′-ジアミノベンジジン四塩酸塩水和物は、免疫組織染色、ウェスタンブロッティング、FISH（蛍光インサイツハイブリダイゼーション）に適しています。DABは、初期白内障における上皮細胞アポトーシスの研究、および常染色体優性多発性嚢胞腎（PKD）の免疫組織化学プロトコールで使用されます。
生物化学的／生理学的作用
DABは、ペルオキシド存在下のペルオキシダーゼ反応で水素供与体として働きます。酸化型DABは、茶色の不溶性複合体を形成します。
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2
保管分類コード
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
D5637-50G:
D5637-H:
D5637-100G-KC:
D5637-1G:
D5637-10G:
D5637-5G:
D5637-100G:
D5637-BULK:
D5637-25G:
2530167:
D5637-VAR:
D5637-EW:
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資料
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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What is the shelf life of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?
1 回答-
役に立ちましたか？
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Do you have a staining protocol for Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?
1 回答-
Dissolve 10 mg in 15 mL of Tris buffered saline, pH 7.6 (Tris-buffered saline tablet, Sigma Product No. T5030 may be used). Add 12 uL of fresh 30% hydrogen peroxide (Sigma Product No. H1009) prior to use. Solution may be filtered through a 0.2 micron filter immediately prior to use if necessary. DAB and hydrogen peroxide concentrations may be adjusted to suit individual applications. The stain can be enhanced with Ni. Make up 0.3% (w/v) NiCl2 in water. Add 1 mL of the NiCl2 stock to 9 mL DAB solution.
役に立ちましたか？
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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Can the solutions of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), be filtered?
1 回答-
DAB solution may be filtered through a 0.2 micron filter immediately prior to use.
役に立ちましたか？
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What is the solution stability of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?
1 回答-
In our experience, solutions of DAB should be made immediately prior to use for best results. If and only if necessary, solutions can be kept in the dark and refrigerated for overnight storage. Based on our Immunology Department’s experience, storing the solution overnight did not give good reproducibility. However, the literature indicates that it is possible to store solutions of DAB at -20°C. DAB dissolved in Tris buffered saline (pH 7.6) at 0.6 mg/ml was reported to be stable for several weeks at 4°C.
役に立ちましたか？
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What is Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), soluble in?
1 回答-
DAB HCl is soluble in water at 50 mg/ml yielding a clear to slightly hazy solution. For consistency, it is important to allow DAB to come to room temperature before making solutions. Water quality is extremely important, so only high quality deionized or distilled water should be used to make DAB solutions.
役に立ちましたか？
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How is Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), shipped and stored?
1 回答-
The product is shipped at ambient temperature. However, it is stored at -20 degrees C for long term storage.
役に立ちましたか？
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