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由来生物
rabbit
結合体
alkaline phosphatase conjugate
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
secondary antibodies
クローン
polyclonal
形状
buffered aqueous solution
テクニック
direct ELISA: 1:7,000
輸送温度
wet ice
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
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詳細
An immunoglobulin has two heavy chains and two light chains connected by disulfide bond.(5) It is a glycoprotein.IgG is a major class of immunoglobulin.(6) Goat IgG has two subclasses- IgG1 and IgG2.(7)
Second antibodies (secondary antibodies) are antibodies raised against other antibodies, typically from another species, called primary antibodies. Primary antibodies are generated with and bind to specific antigens to create antigen antibody complexes which may be detected in a variety of immunochemical and immunohistochemical configurations involving soluble or insoluble signaling molecules. The immunodetection system depends upon the binding of a signal competent secondary antibody to a primary antibody:antigen complex.
The suitability of any secondary antibody to a specific immunodetection application is dependent upon its signal development system configuration. Signal development systems are based upon the chemical conjugation of a signal mediator to the secondary antibody. Major secondary antibody signal systems are color (chromogenic), chemiluminescence or fluorescence based and involve the use of enzymes or high affinity binary systems such as biotin:avidin.
The suitability of any secondary antibody to a specific immunodetection application is dependent upon its signal development system configuration. Signal development systems are based upon the chemical conjugation of a signal mediator to the secondary antibody. Major secondary antibody signal systems are color (chromogenic), chemiluminescence or fluorescence based and involve the use of enzymes or high affinity binary systems such as biotin:avidin.
特異性
Antiserum is determined to be immunospecific for goat IgG by immunoelectrophoresis (IEP), prior to conjugation, versus goat IgG and normal goat serum.
免疫原
Antiserum is produced in rabbit using purified goat IgG as the immunogen
アプリケーション
Anti-Goat IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit has been used in western blotting.
Anti-Goat IgG (whole molecule)-Alkaline Phosphatase antibody produced in rabbit has been used in immunoblotting and enzyme-linked immunosorbent assay (ELISA).
Immunocytochemistry was performed on cells fixed in 4% paraformaldhyde for 15 minutes using alkaline phosphatase-conjugated rabbit anti-goat IgG diluted 1:1000 in TBS as the secondary antibody.
生物化学的／生理学的作用
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to the fetus through the placenta that is vital for immune defense of the neonate against infections.
Immunoglobulin G (IgG) mainly helps in immune defense.(5) IgG participates in hypersensitivity type II and type III.
物理的形状
0.05M Tris溶液 (pH 8.0, 10mMグリシン, 1%BSA, 1mM MgCl2, 50%グリセロ-ル, 15mMアジ化ナトリウム含有)。
保管および安定性
Store at 2-8°C.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
A7650-1ML:
A7650-BULK:
A7650-.25ML:
A7650-VAR:
A7650-.5ML:
A7650-5X1ML:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Yalan Li et al.
Antioxidants & redox signaling, 38(1-3), 115-136 (2022-06-17)
Aims: Noise damage to auditory hair cells is associated with oxidative stress and mitochondrial dysfunction. This study aimed to investigate the possible effect of sestrin 2 (SESN2), an endogenous antioxidant protein, on noise-induced hearing loss (NIHL) and the underlying mechanisms.
MCMV triggers ROS/NLRP3-associated inflammasome activation in the inner ear of mice and cultured spiral ganglion neurons, contributing to sensorineural hearing loss
Zhuang W, et al.
International Journal of Molecular Medicine, 41(6), 3448-3456 (2018)
Placental transfer of IgG subclasses in a Japanese population.
Hashira S, et al.
Pediatrics International : Official Journal of the Japan Pediatric Society, 42(4), 337-342 (2000)
The significance of complement in proliferative vitreoretinopathy.
Grisanti S, et al.
Investigative Ophthalmology & Visual Science, 32(10), 2711-2717 (1991)
Wei Zhuang et al.
International journal of molecular medicine, 41(6), 3448-3456 (2018-03-08)
Congenital cytomegalovirus (CMV) infection is the most common infectious cause of sensorineural hearing loss in children. While the importance of CMV‑induced SNHL has been described, the mechanisms underlying its pathogenesis and the role of inflammatory responses remain elusive. The present
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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