T3934
TRI reagent®
LS, For processing fluid samples such as cell suspensions, CSF, and amniotic fluid.
Sinonimo/i:
TRI Reagent® reagente per l’isolamento dell’RNA
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100 ML
345,00 €
200 ML
592,00 €
345,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
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Cambia visualizzazione
100 ML
345,00 €
200 ML
592,00 €
About This Item
345,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
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impiego
0.75 mL sufficient for 0.25 mL fluid samples
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Categorie correlate
Descrizione generale
TRI Reagent LS is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA, and protein from liquid samples of human, animal, plant, yeast, bacterial, and viral origin.
A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA, and protein. This procedure is an adaptation of the singlestep method reported by Chomczynski and Sacchi for total RNA isolation, and permits fast and efficient processing of liquid samples. TRI Reagent LS performs well with large or small sample volumes, and many samples can be simultaneously extracted.
TRI Reagent LS is a mixture of guanidine thiocyanate and phenol in a monophase solution. When a biological sample is homogenized or lysed with it, and chloroform or 1-bromo-3-chloropropane is added, the mixture separates into 3 phases: an aqueous phase containing RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. 0.75 ml of TRI Reagent LS processes 0.25 ml of a liquid sample such as amniotic fluid.
This is one of the most effective methods for isolating total RNA from fresh samples in only one hour. The procedure is very effective for isolating RNA molecules of all types from 0.1-15 kb in length. The resulting RNA is intact with little or no contaminating DNA and protein.
A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA, and protein. This procedure is an adaptation of the singlestep method reported by Chomczynski and Sacchi for total RNA isolation, and permits fast and efficient processing of liquid samples. TRI Reagent LS performs well with large or small sample volumes, and many samples can be simultaneously extracted.
TRI Reagent LS is a mixture of guanidine thiocyanate and phenol in a monophase solution. When a biological sample is homogenized or lysed with it, and chloroform or 1-bromo-3-chloropropane is added, the mixture separates into 3 phases: an aqueous phase containing RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. 0.75 ml of TRI Reagent LS processes 0.25 ml of a liquid sample such as amniotic fluid.
This is one of the most effective methods for isolating total RNA from fresh samples in only one hour. The procedure is very effective for isolating RNA molecules of all types from 0.1-15 kb in length. The resulting RNA is intact with little or no contaminating DNA and protein.
Applicazioni
Il Tri reagent consiste in una versione perfezionata del reagente per l'estrazione dell'RNA totale a singolo passaggio sviluppato da Chomczynski. Il metodo di isolamento dell'RNA basato su questo reagente è ampiamente utilizzato e di comprovata affidabilità per le successive applicazioni dell′RNA È ideale per l'isolamento rapido, economico ed efficiente dell'RNA totale o per l'isolamento simultaneo di RNA, DNA e proteine da campioni di origine umana, animale, vegetale, di lievito, batterici e virali.
The reagent has been used for isolating total RNA.[4] The resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assay, cloning, and PCR.
Caratteristiche e vantaggi
- Isolamento dell'RNA facilmente scalabile
- Funziona con campioni di diversa origine: umana, vegetale, di lievito, batterica o virale
- Ha una resa migliore rispetto ai tradizionali metodi guanidina tiocianato/cloruro di cesio
Note legali
TRI Reagent is a registered trademark of Molecular Research Center, Inc.
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Danger
Indicazioni di pericolo
Classi di pericolo
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
Organi bersaglio
Nervous system,Kidney,Liver,Skin
Rischi supp
Codice della classe di stoccaggio
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
174.2 °F - closed cup
Punto d’infiammabilità (°C)
79 °C - closed cup
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Articoli
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
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