This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein on homogenization or lysis of tissue sample. After adding chloroform or 1-bromo3-chloropropane and centrifuging, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. Please see the link below to review further information as well as review specific protocols:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/294/543/t9424bulletin.pdf
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| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
25 mL | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 167,00 € |
100 mL | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 499,00 € |
Informazioni su questo articolo
167,00 €
form
liquid
Quality Segment
usage
mL sufficient for 107 cells, mL sufficient for 100 mg tissue
impurities
phenol
General description
Application
Features and Benefits
- Isolamento dell'RNA facilmente scalabile
- Funziona con campioni di diversa origine: umana, vegetale, di lievito, batterica o virale
- Ha una resa migliore rispetto ai tradizionali metodi guanidina tiocianato/cloruro di cesio
Legal Information
1 of 1
Questo articolo | |||
|---|---|---|---|
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| form liquid | form - | form - | form - |
| impurities phenol | impurities - | impurities - | impurities - |
| usage mL sufficient for 107 cells, mL sufficient for 100 mg tissue | usage mL sufficient for 107 cells, mL sufficient for 100 mg tissue (or) | usage 0.75 mL sufficient for 0.25 mL fluid samples | usage 0.75 mL sufficient for 0.25 mL blood derivatives |
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signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
target_organs
Nervous system,Kidney,Liver,Skin
supp_hazards
Classe di stoccaggio
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 2
flash_point_f
174.2 °F - closed cup
flash_point_c
79 °C - closed cup
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Excuse me, what is the principle of trizol reagent to separate DNA and RNA?
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