P9172

Pyronin Y

for NA electrophoresis

• Sinonimo/i: Pyronin Y, Pyronin G, Pyronin J
• Formula empirica (notazione di Hill): C₁₇H₁₉ClN₂O
• Numero CAS: 92-32-0
• Peso molecolare: 302.80
• Colour Index: 45005
• Beilstein: 3795789
• Numero CE: 202-147-8
• Numero MDL: MFCD00011725
• Codice UNSPSC: 12171500
• ID PubChem: 57654569
• NACRES: NA.52

Proprietà

• Grado: for molecular biology
• Livello qualitativo: 200
• Forma fisica: powder
• Composizione: Dye content, ≥45%
• tecniche: nucleic acid detection: suitable
• Punto di fusione: 250-260 °C (lit.)
• Solubilità: water: 10 mg/mL, dark red
• Compatibilità: suitable for nucleic acid staining
• Temperatura di conservazione: room temp
• Stringa SMILE: [Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C
• InChI: 1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1; INCIMLINXXICKS-UHFFFAOYSA-M

Descrizione

Applicazioni

Pyronin Y has been used:

• in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)
• as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)
• staining of CD34⁺ cells

Azioni biochim/fisiol

Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.

Nota sulla preparazione

This product is soluble in water 1 mg/ml, yielding a clear dark red solution.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)