M8884

Methyl Green

~85% purity, powder

• Sinonimo/i: Ethyl Green
• Formula condensata: C₂₇H₃₅BrClN₃ · ZnCl₂
• Numero CAS: 7114-03-6
• Peso molecolare: 653.24
• Colour Index: 42590
• Beilstein: 3914106
• Numero CE: 230-415-4
• Numero MDL: MFCD00151094
• Codice UNSPSC: 12171500
• ID PubChem: 24897293
• NACRES: NA.47

Proprietà

• product name: Methyl Green, zinc chloride salt, ~85%
• Livello qualitativo: 200
• Saggio: ~85%
• Forma fisica: powder
• Composizione: Dye Content, ≥83%
• Colore: red to very dark red
• pH: 0.2-1.8, yellow to blue
• Punto di fusione: >300 °C (lit.)
• Solubilità: water: 1 mg/mL
• εmax: >11100.00 at 417-425 nm in water at 0.005 g/L; >16000 at 311-319 nm in water at 0.005 g/L; >60000 at 626-634 nm in water at 0.005 g/L
• applicazioni: diagnostic assay manufacturing; hematology; histology
• Temperatura di conservazione: room temp
• Stringa SMILE: [Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C
• InChI: 1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4; RRKSQWOQZFIQKW-UHFFFAOYSA-J

Descrizione

Descrizione generale

Methyl green is a synthetic, triphenylmethane-type cationic dye related to ethyl green mainly used as DNA stain in histochemistry. It possesses aniline rings with different degrees of methylation. The dye binds specifically to the A-T rich regions of the major groove of DNA with the help of two positive charges. Methyl green in combination with pyronin is extensively used to stain RNA and plasma cells in tissue sections and cytologic preparations.

Applicazioni

Methyl green is suitable as a nuclear counterstain in enzyme histochemistry, immunostaining, and in situ hybridization as the bluish-green color will be in contrast with red, purple, brown, and black products of histochemical reactions. It is also suitable as a tracking dye in Acid-polyacrylamide gel electrophoresis to separate gliadins. It has been used in the histochemical staining for the apoptosis examination of kidney tissue and as a nuclear stain for labeling embryos.

Qualità

Material may be a mixture of monobromo-monochloro and dichloro ZnCl₂ salts. Halogen contents listed on label.

Risultati analitici

Dye content based on molecular weight of monobromo-monochloro ZnCl₂ salt (653.2).

Informazioni sulla sicurezza

• Pittogrammi: GHS05,GHS07,GHS09
• Avvertenze: Danger
• Indicazioni di pericolo: H314,H335,H411
• Consigli di prudenza: P260 - P271 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Classi di pericolo: Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3
• Organi bersaglio: Respiratory system
• Codice della classe di stoccaggio: 8A - Combustible corrosive hazardous materials
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Dispositivi di protezione individuale: dust mask type N95 (US), Eyeshields, Gloves