P3895
Phosphatase, Alkaline from human placenta
≥10 units/mg solid (in glycine buffer)
Sinonimo/i:
Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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About This Item
Prodotti consigliati
Forma fisica
solid
Attività specifica
≥10 units/mg solid (in glycine buffer)
N° accesso UniProt
Temperatura di conservazione
−20°C
Informazioni sul gene
human ... ALPP(250)
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Applicazioni
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It is routinely used to dephosphorylate proteins and nucleic acids. It may be used for protein labeling when high sensitivity is required. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase.
Azioni biochim/fisiol
Alkaline phosphatase dephosphorylates proteins and nucleic acids. Human placental alkaline phosphatase (PLAP) has an unique residue, Glu-429. It is an allosteric enzyme that is uncompetitively inhibited by L-Phe, L-Trp, and L-Leu. PLAP is post-translationally modified. Phosphatidylinositol-specific phospholipase C may be involved in the release of PLAP. PLAP may have a role in cell division and may be involved in the transfer of maternal IgG to the fetus.
Definizione di unità
One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 10.4 at 37 °C.
Inibitore
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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