Significant lot-to-lot variability in potency is quite normal for this product. It is a somewhat crude phenol extract; it is not chromatographically purified. That is, a chromatographically purified LPS such as Product L3012 would be expected to have better lot-to-lot consistency. Note that for both this product and L3012, the potency specification is greater than or equal to 500,000 Endotoxin units per milligram (EU/mg); there is a lower limit, but there is no upper limit.
L2630
Lipopolisaccaridi
Lipopolysaccharides from Escherichia coli O111:B4
purified by phenol extraction
Sinonimo/i:
LPS
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Scegli un formato
10 MG
70,60 €
25 MG
133,00 €
100 MG
337,00 €
Scegli un formato
Cambia visualizzazione
10 MG
70,60 €
25 MG
133,00 €
100 MG
337,00 €
About This Item
Prodotti consigliati
Origine biologica
Escherichia coli (O111:B4)
Livello qualitativo
Stato
lyophilized powder
Purificato mediante
phenol extraction
tecniche
ELISA: suitable
cell based assay: suitable
ligand binding assay: suitable
Impurezze
≤3.00% protein (Lowry-TCA)
Colore
off-white to faint yellow
Solubilità
water: soluble
applicazioni
cell analysis
clinical research
life science and biopharma
Condizioni di spedizione
ambient
Temperatura di conservazione
2-8°C
Cerchi prodotti simili? Visita Guida al confronto tra prodotti
Descrizione generale
I lipopolisaccaridi sono glicolipidi, quindi composti da carboidrati legati ad acidi grassi.[1] I lipopolisaccaridi (LPS) sono componenti caratteristici della parete cellulare dei batteri Gram negativi. Una tipica molecola di LPS contiene il lipide A (un fosfolipide a base di glucosamina), un oligosaccaride core relativamente corto e un polisaccaride distale (O-antigene). Gli LPS contribuiscono all′integrità della membrana esterna e proteggono la cellula contro l′azione dei sali biliari e degli antibiotici lipofili. Stimolano le cellule dell′ospite tramite TLR2 e TLR4 (recettori toll-like). Questa stimolazione determina la generazione di varie citochine proinfiammatorie, quali il TNFα (fattore di necrosi tumorale), l′IL-1 (interleuchina 1) e l′IL-6.[2][3]
Questo prodotto viene estratto con fenolo dal sierotipo di Escherichia coli O111:B4. Il ceppo di origine proviene da una collezione privata. Questo sierotipo di LPS è stato utilizzato per stimolare le cellule B e indurre la NOS (ossido nitrico sintasi) negli epatociti umani.
Questo prodotto viene estratto con fenolo dal sierotipo di Escherichia coli O111:B4. Il ceppo di origine proviene da una collezione privata. Questo sierotipo di LPS è stato utilizzato per stimolare le cellule B e indurre la NOS (ossido nitrico sintasi) negli epatociti umani.
Applicazioni
I lipopolisaccaridi (LPS) estratti da Escherichia coli O111:B4 sono stati usati per:
- la stimolazione dei macrofagi;[4][5]
- il precondizionamento con LPS nei topi;[6]
- indurre danno renale acuto nei topi;[7]
- indurre la sindrome da distress respiratorio acuto (ARDS) nei topi per studiare l'effetto antinfiammatorio di esosomi derivati da cellule staminali mesenchimali di midollo osseo (BMSC);[8]
- indurre uno stato iperinfiammatorio in topi infettati con il coronavirus responsabile della sindrome respiratoria acuta grave (SARS-CoV-2)[9]
I lipopolisaccaridi (LPS) sono componenti caratteristici della parete cellulare dei batteri gram negativi. Il LPS e la sua frazione lipide A stimolano le cellule del sistema immunitario innato attraverso il recettore Toll-like 4 (TLR4), un elemento della famiglia delle proteine recettoriali Toll-like, che riconosce i profili molecolari associati ai patogeni (PAMP) comuni.
Azioni biochim/fisiol
La funzione dei lipopolisaccaridi (LPS) che compongono la membrana esterna è quella di renderla impermeabile alle molecole idrofobiche, come gli antibiotici, che rende i batteri Gram-negativi resistenti per natura. Inoltre gli LPS svolgono un ruolo nelle interazioni batterio-ospite che regolano le risposte da parte del sistema immunitario dell'ospite.[1]
Nota sulla preparazione
Il prodotto è solubile in acqua (5 mg/ml) o in terreno di coltura cellulare (1 mg/ml) e produce una soluzione opaca di colore giallino. Una soluzione più concentrata (20 mg/ml), ma sempre opaca, è stata ottenuta in soluzione acquosa salina dopo la miscelazione con vortex e il riscaldamento a 70-80 oC. I lipopolisaccaridi sono molecole che formano micelle in ogni solvente. Si osservano soluzioni opache in acqua e tampone fosfato salino. I solventi organici non producono soluzioni più trasparenti. Il metanolo produce una sospensione torbida con particelle galleggianti, mentre l′acqua produce una soluzione omogeneamente opaca.
Altre note
Per acquisire un quadro completo della nostra ampia selezione di lipopolisaccaridi disponibili per la vostra ricerca, vi invitiamo a consultare la nostra pagina dedicata ai carboidrati.
Per ulteriori informazioni sulla nostra gamma di Composti biochimici, compilare questo modulo.
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Acute Tox. 2 Oral
Codice della classe di stoccaggio
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Scegli una delle versioni più recenti:
Certificati d'analisi (COA)
Lot/Batch Number
Non trovi la versione di tuo interesse?
Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Glycolipid biosurfactants: Biosynthesis and related potential applications in food industry
Ashby R D, et al.
Journal of Separation Science, 307-334 (2023)
Lei Li et al.
Shock (Augusta, Ga.), 51(6), 770-779 (2018-12-19)
Long noncoding RNAs (lncRNAs), once thought to be transcriptional noise, have been recently shown to regulate a variety of biological processes. However, their roles in the inflammatory response are largely unexplored. In this study, we performed high-throughput sequencing to identify
Corticotropin releasing factor receptor 1 (CRF1) and CRF2 agonists exert an anti-inflammatory effect during the early phase of inflammation suppressing LPS-induced TNF-alpha release from macrophages via induction of COX-2 and PGE2.
Tsatsanis C et al.
Journal of Cellular Physiology, 210, 774-774 (2007)
Tingting Wang et al.
Journal of gastroenterology, 54(9), 804-818 (2019-05-01)
Ulcerative colitis (UC) is characterized by chronic inflammation in the colon and epigenetic factors underlying the occurrence. Circular RNAs (circRNAs) have been under intensive focus due to the circular construct and gene-regulating functions. However, the changes and roles of circRNAs
Aya Yanagawa et al.
PloS one, 12(11), e0185370-e0185370 (2017-11-10)
Behavioral resistance protects insects from microbial infection. However, signals inducing insect hygiene behavior are still relatively unexplored. Our previous study demonstrated that olfactory signals from microbes enhance insect hygiene behavior, and gustatory signals even induce the behavior. In this paper
Articoli
Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.
-
When I compared COAs of two batches of LPS (L2630) we have been using, I realized the potency of the newer one is 10-fold higher than the older one (30000000 and 3000000 EU/mg). Is it common for LPS that different lots have such different potency?
1 risposta-
Utile?
-
-
How can I determine the shelf life / expiration / retest date of this product?
1 risposta-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfUtile?
-
-
How is shipping temperature determined? And how is it related to the product storage temperature?
1 risposta-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Utile?
-
-
Is there any harm in filter sterilizing a solution with this LPS before using it in cell culture or animals? Meaning does it change or lose potency if it is sterile filtered say through a 0.2 micron filter?
1 risposta-
Sterile filtration should not affect the concentration or properties of the solution if the LPS is fully dissolved, but please keep in mind that this is a research-grade material that has not been best tested on animals. For a cell culture grade material that has already been γ-irradiated, please consider the following: https://www.sigmaaldrich.com/product/sigma/l4391
Utile?
-
-
I received LPS L2630 at Room Temperature. Is it ok to keep it at Room Temperature before reconstitution, although recommendations are to store it between 2-8 C?
1 risposta-
This product ships routinely at ambient temperature and is stable up to 2 weeks to allow for transit time. The item is to be stored at 2-8°C when received.
Please see the link below to review the product storage and shipping guidelines:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/232/426/storage-transport-conditions-ms.pdfUtile?
-
-
Is product L2630 suitable for use in mammalian cell culture?
1 risposta-
Specific testing or validation for the use of product L2630 in mammalian cell culture has not been conducted.
Utile?
-
-
Hi i recieved L2630 10mg Lipopolysaccharides from Escherichia coli O111:B4 i will used in experimental work. how i will dilute it
1 risposta-
The product is soluble in water (5 mg/mL) or cell culture medium (1 mg/mL). Please see the link below to review additional preparation and storage information:
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharidesUtile?
-
-
What is the purity level of this product ? is it ultrapure? 100% pure LPS ?
1 risposta-
This product is not tested for purity. However, it is tested for potency on a lot-specific basis.
Kindly review a sample CoA accessible at this link: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/L2630-VAR0000271356.pdf
Utile?
-
-
Once a solution is developed by dissolving product Lipopolysaccharides from Escherichia coli O111:B4 in water, what is the storage protocol and and how long will it remain usable?
1 risposta-
Please see the following link describing our LPS products: https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides
STORAGE/STABILITY
Solutions at 1 mg/mL in buffer or culture medium are stable for approximately one month at 2-8 °C. Frozen aliquots can be stored up to 2 years. Repeated freeze/thaw cycles are not recommended. Solutions should be stored in silanized containers, since LPS can bind to plastics and certain types of glass (especially at concentrations of <0.1 mg/mL). If the LPS concentration is >1 mg/mL, adsorption to the sides of the vial is negligible. If glass containers are used, solutions should be vortexed for at least 30 minutes to redissolve the adsorbed product.Utile?
-
-
How concentrated a stock solution can I make of Product L2630, Lipopolysaccarides (LPS)?
1 risposta-
One can try to make a 20 mg/mL solution at 70-80°C in saline, with vortexing and warming. However, the solution will be hazy, because of the nature of LPS to form micelles in solutions like water or PBS.
Utile?
-
Filtri attivi
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.
