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L6529

Sigma-Aldrich

Lipopolisaccaridi

γ-irradiated, BioXtra, suitable for cell culture

Sinonimo/i:

LPS

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1 MG
84,40 €

84,40 €

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84,40 €

About This Item

Numero CAS:
93572-42-0
Numero CE:
297-473-0
Numero MDL:
MFCD00164401
Codice UNSPSC:
12352211
NACRES:
NA.28

84,40 €

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Origine biologica

Escherichia coli (O55:B5)

Livello qualitativo

300

Sterilità

γ-irradiated

Nome Commerciale

BioXtra

Stato

lyophilized powder

Purificato mediante

gel-filtration chromatography

tecniche

cell culture | mammalian: suitable

Impurezze

<3% Protein (Lowry)

Solubilità

H2O: 5 mg/mL, faintly hazy to hazy

Temperatura di conservazione

2-8°C

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Categorie correlate

Descrizione generale

Lipopolysaccharides (LPS) are complex glycolipids that contain several components. Enterobacteriaceae, such as E. coli, have highly conserved lipid A linked to the antigenic O-polysaccharide. Hydrophobic lipid A forms the outer most component that plays a role in the biological properties attributed to endotoxic LPS. A short core oligosaccharide (core OS) is found to be attached to lipid A. Enterobacteria contain an antigenic, repeat-structure O-polysaccharide (O-PS) attached to the distal core OS to form smooth LPS. O-PS in E. coli contains ∼170 distinct antigens that interact with the host immune system. Based on the core OS structures in E. coli, they have been designated K-12 and R1 to R4.[1][2]

Applicazioni

I lipopolisaccaridi (LPS) sono componenti caratteristici della parete cellulare dei batteri gram negativi. Il LPS e la sua frazione lipide A stimolano le cellule del sistema immunitario innato attraverso il recettore Toll-like 4 (TLR4), un elemento della famiglia delle proteine recettoriali Toll-like, che riconosce i profili molecolari associati ai patogeni (PAMP) comuni.
LPS (lipopolysaccharide) from Escherichia coli 055:B5 has been used:
  • to stimulate human PBMC to secrete cytokine.[3]
  • to stimulate wild type embryonic fibroblasts and mutants for the induction of phosphorylation of p56[4]
  • in the measurement of tumor necrosis factor-α levels and nuclear factor-κB p65 activities after stimulation[5]
  • in the stimulation of hamster lymphocytes in vitro[6]
  • to induce activated dendritic morphology in murine macrophage cell line in a study[7]

Azioni biochim/fisiol

LPS (lipopolysaccharide) is a major constituent of the outer membrane of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens.[8][9] The LPS confers antigenic and endotoxic characters to the bacteria. They are also important in resistance to infection by phages.[10]

Ricostituzione

Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.

Altre note

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Pittogrammi

Skull and crossbones
GHS06

Avvertenze

Danger

Indicazioni di pericolo

H300

Classi di pericolo

Acute Tox. 2 Oral

Codice della classe di stoccaggio

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
0000371444
0000371444
0000200756
0000160286
0000200756

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Natalie M Otto et al.
Critical care (London, England), 12(4), R107-R107 (2008-08-20)
Hyperglycemia is associated with negative outcomes in various settings of critical illness; infectious complications, especially, seem to be increased. On the other hand, intensive insulin therapy (IIT) has been shown to improve outcome in clinical trials. Whether normoglycemia itself or
PURIFICATION AND CHARACTERIZATION OF THE LIPID A COMPONENT OF THE LIPOPOLYSACCHARIDES FROM ESCHERICHIA COLI.
A J BURTON et al.
Biochemistry, 3, 411-418 (1964-03-01)
Cheng Yang Ng et al.
Scientific reports, 7(1), 13683-13683 (2017-10-24)
Cytosolic phospholipase A
Katerina Akassoglou et al.
Proceedings of the National Academy of Sciences of the United States of America, 101(17), 6698-6703 (2004-04-21)
In multiple sclerosis, in which brain tissue becomes permeable to blood proteins, extravascular fibrin deposition correlates with sites of inflammatory demyelination and axonal damage. To examine the role of fibrin in neuroinflammatory demyelination, we depleted fibrin in two tumor necrosis
D E Heinrichs et al.
Molecular microbiology, 30(2), 221-232 (1998-10-29)
Bacterial lipopolysaccharides (LPS) are unique and complex glycolipids that provide characteristic components of the outer membranes of Gram-negative bacteria. In LPS of the Enterobacteriaceae, the core oligosaccharide links a highly conserved lipid A to the antigenic O-polysaccharide. Structural diversity in
Visualizza tutti i documenti correlati

Articoli

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Domande

1–2 di 2 domande  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 risposta

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Utile?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 risposta

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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