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L2637

Sigma-Aldrich

Lipopolisaccaridi

purified by gel-filtration chromatography

Sinonimo/i:

LPS

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5 MG
261,00 €
10 MG
598,00 €
25 MG
1.230,00 €

261,00 €

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Cambia visualizzazione
5 MG
261,00 €
10 MG
598,00 €
25 MG
1.230,00 €

About This Item

Numero CAS:
93572-42-0
Numero CE:
297-473-0
Numero MDL:
MFCD00164401
Codice UNSPSC:
12352201
NACRES:
NA.25

261,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Richiedi un ordine bulk

Origine biologica

Escherichia coli (O55:B5)

Livello qualitativo

200

Stato

lyophilized powder

Purificato mediante

gel-filtration chromatography

Impurezze

<3% Protein

Colore

white to faint gray

Solubilità

water: soluble

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

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Categorie correlate

Descrizione generale

This product is extracted from E. coli serotype O55:B5 and purified by gel filtration. The source strain is CDC 1644-70. The LPS O55:B5 has been used to stimulate human peritoneal macrophages at 1 ng/ml and to stimulate equine peritoneal macrophages at 1-100 ng/ml.

Applicazioni

I lipopolisaccaridi (LPS) sono componenti caratteristici della parete cellulare dei batteri gram negativi. Il LPS e la sua frazione lipide A stimolano le cellule del sistema immunitario innato attraverso il recettore Toll-like 4 (TLR4), un elemento della famiglia delle proteine recettoriali Toll-like, che riconosce i profili molecolari associati ai patogeni (PAMP) comuni.

Azioni biochim/fisiol

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Nota sulla preparazione

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Altre note

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Pittogrammi

Skull and crossbones
GHS06

Avvertenze

Danger

Indicazioni di pericolo

H300

Classi di pericolo

Acute Tox. 2 Oral

Codice della classe di stoccaggio

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
0000431738
0000431738
0000371463
0000371465
0000371464

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John Bienenstock et al.
PloS one, 8(10), e76236-e76236 (2013-10-08)
Human milk oligosaccharides (HMO) are being studied by different groups exploring a broad range of potential beneficial effects to the breastfed infant. Many of these effects have been attributed to a growth promotion effect on certain gut organisms such as
Yunru Ren et al.
Water research, 154, 153-161 (2019-02-21)
Aerosolized reclaimed water can cause inflammatory responses in lung after inhalation, and endotoxin has been identified as the main inducer. Since the effects of disinfection treatments on endotoxins had conflicting results, this study explored the changes of endotoxin activity and
Francisco J Quintana et al.
PloS one, 3(10), e3509-e3509 (2008-10-24)
B-cells integrate antigen-specific signals transduced via the B-cell receptor (BCR) and antigen non-specific co-stimulatory signals provided by cytokines and CD40 ligation in order to produce IgG antibodies. Toll-like receptors (TLRs) also provide co-stimulation, but the requirement for TLRs to generate
Zili Zhai et al.
Inflammation, 36(2), 346-354 (2012-10-12)
The nitrone spin trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO) is commonly used to study free radicals. Due to its free radical trapping properties, DMPO is thought to reduce free radial-mediated oxidative damage and other related cellular responses. The purpose of this study
J Vier et al.
Cell death & disease, 7, e2103-e2103 (2016-02-20)
Neutrophil granulocytes are innate effector cells of the first line of defense against pyogenic bacteria. Neutrophil lifespan is short, is prolonged by pro-inflammatory stimuli, controls functionality of the cells and can determine tissue damage. Experimental analysis of primary neutrophils is
Visualizza tutti i documenti correlati

Articoli

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Contenuto correlato

Lipopolysaccharides

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

Domande

1–4 di 4 domande  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 risposta

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Utile?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 risposta

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Utile?

  3. What is the molecular weight of this product ?

    1 risposta

    1. Lipopolysaccharides(LPS) are heterogeneous and do not have fully defined molecular weights. In the presence of strong surface active agents, and in the absence of divalent cations, LPS will have molecular weights in the range of 10?20 kDa. Please see the link below to review additional information regarding the nature of LPS, including molecular weight variability:
      https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/research-and-disease-areas/cell-signaling/lipopolysaccharides

      Utile?

  4. Can I use DPBS to dilute? or just use water?

    1 risposta

    1. This product is tested for solubility at 5 mg/mL in water. Aqueous buffers such as DPBS are expected to be suitable as well. Pease see the link below to review a reference that may be helpful:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5685187/

      Utile?

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