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L5293

Sigma-Aldrich

Lipopolisaccaridi

Ready Made solution, 1 mg/mL

Sinonimo/i:

LPS

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2 ML
311,00 €

311,00 €

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Cambia visualizzazione
2 ML
311,00 €

About This Item

Numero CAS:
93572-42-0
Numero MDL:
MFCD00164401
Codice UNSPSC:
12352201
NACRES:
NA.25

311,00 €

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Origine biologica

Escherichia coli (O111:B4)

Livello qualitativo

200

Stato

aqueous solution

Concentrazione

1 mg/mL

Colore

colorless to faint yellow

Solubilità

water: soluble

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

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Applicazioni

I lipopolisaccaridi (LPS) sono componenti caratteristici della parete cellulare dei batteri gram negativi. Il LPS e la sua frazione lipide A stimolano le cellule del sistema immunitario innato attraverso il recettore Toll-like 4 (TLR4), un elemento della famiglia delle proteine recettoriali Toll-like, che riconosce i profili molecolari associati ai patogeni (PAMP) comuni.
Lipopolysaccharides from Escherichia coli O111:B4 has been used:
  • to stimulate an inflammatory response in porcine liver tissues in-vivo[1] and piglet ileum tissues ex vivo[2]
  • to stimulate Ex vivo natural killer(NK) cells[3] and hepatic B cells[4]
  • to induce arthritis in a standard rabbit model[5]

Azioni biochim/fisiol

Lipopolysaccharides (LPS) are distributed on the outer layer of the membrane and in non-capsulated strains, present on the cell surface. LPS maintains the outer membrane integrity. In addition, it protects the cell from bile salts stress and lipophilic antibiotics.[6]

Nota sulla preparazione

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Altre note

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificati d'analisi (COA)

Lot/Batch Number
0000408042
0000408042
0000294757
0000294757
0000288999

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

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NKG2D is critical for NK cell activation in host defense against Pseudomonas aeruginosa respiratory infection
Wesselkamper S, et al.
Journal of immunology (Baltimore, Md. : 1950), 181(8), 5481-5489 (2008)
Effect of maternal seaweed extract supplementation on suckling piglet growth, humoral immunity, selected microflora, and immune response after an ex vivo lipopolysaccharide challenge
Leonard SG, et al.
Journal of Animal Science, 90(2), 505-514 (2012)
Effects of dietary beta-glucans supplementation on cytokine expression in porcine liver
Ryan M, et al.
Journal of Animal Science, 90(suppl-4), 40-42 (2012)
6 Analysis of Lipopolysaccharides of Gram-Negative Bacteria
Methods in Microbiology, 18(5), 157-207 (1985)
Imaging of normal and pathologic joint synovium using nonlinear optical microscopy as a potential diagnostic tool
Tiwari N, et al.
Journal of Leukocyte Biology, 15(5), 056001-056001 (2010)
Visualizza tutti i documenti correlati

Articoli

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Contenuto correlato

Lipopolysaccharides

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

Domande

1–5 di 5 domande  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 risposta

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Utile?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 risposta

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Utile?

  3. What is recommended for the storage of longer duration once opened? Is it better to keep at 2°C-8°C for 1-2 years or to freeze it (-80°C or -20°C) and thaw it gently when needed?

    1 risposta

    1. This product is stable at 2-8°C at least until the assigned retest date, provided that no contaminants have been introduced. The recommended retest date is lot specific and reported in the product Certificate of Analysis. The stability of the product in dilute form or beyond the assigned retest date has not been determined. However, LPS products prepared from the powder form may be stored in 1 mg/mL aliquots for up to 2 years at -20°C. The storage of solutions at -80°C has not been determined. Please see the link below to review additional information regarding the stability and preparation of Lipopolysaccharides:
      https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides

      Utile?

  4. What solvent is the product L5293-2ML dissolved in?

    1 risposta

    1. The solvent information for this product is proprietary, but it is acknowledged to contain water, while the remaining components remain undisclosed.

      Utile?

  5. Bonjour, Comment ce LPS a t'il été extrait / Purifié ?

    1 risposta

    1. This product is a sterile filtered solution. The extraction method for this material is considered proprietary, however it is chromatographically purified.

      Utile?

Recensioni

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