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L4005

Sigma-Aldrich

Lipopolisaccaridi

purified by trichloroacetic acid extraction

Sinonimo/i:

LPS

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100 MG
381,00 €
500 MG
1.560,00 €

381,00 €


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Cambia visualizzazione
100 MG
381,00 €
500 MG
1.560,00 €

About This Item

Numero CAS:
Numero CE:
Numero MDL:
Codice UNSPSC:
12352201
NACRES:
NA.25

381,00 €


Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Richiedi un ordine bulk

Origine biologica

Escherichia coli (O55:B5)

Livello qualitativo

Stato

lyophilized powder

Purificato mediante

trichloroacetic acid extraction

Impurezze

1-10% Protein (Lowry)

Colore

white to yellow

Solubilità

water: soluble

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

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Descrizione generale

This product is TCA extracted from E. coli serotype O55:B5. The source strain is CDC 1644-70. The LPS O55:B5 has been used to stimulate human peritoneal macrophages at 1 ng/ml and to stimulate equine peritoneal macrophages at 1-100 ng/ml.

Applicazioni

I lipopolisaccaridi (LPS) sono componenti caratteristici della parete cellulare dei batteri gram negativi. Il LPS e la sua frazione lipide A stimolano le cellule del sistema immunitario innato attraverso il recettore Toll-like 4 (TLR4), un elemento della famiglia delle proteine recettoriali Toll-like, che riconosce i profili molecolari associati ai patogeni (PAMP) comuni.

Azioni biochim/fisiol

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Nota sulla preparazione

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Altre note

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Pittogrammi

Skull and crossbones

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Acute Tox. 2 Oral

Codice della classe di stoccaggio

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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A Fernandez-Bustamante et al.
Respiratory physiology & neurobiology, 183(2), 149-158 (2012-06-26)
The regional distribution of inflammation during acute lung injury (ALI) is not well known. In an ovine ALI model we studied regional alveolar inflammation, surfactant composition, and CT-derived regional specific volume change (sVol) and specific compliance (sC). 18 ventilated adult
O Schulz et al.
Mucosal immunology, 7(4), 892-904 (2013-12-19)
Lymphoid organ hypertrophy is a hallmark of localized infection. During the inflammatory response, massive changes in lymphocyte recirculation and turnover boost lymphoid organ cellularity. Intriguingly, the exact nature of these changes remains undefined to date. Here, we report that hypertrophy
Yu-Yuan Chen et al.
PloS one, 9(12), e115232-e115232 (2014-12-18)
Invertebrates rely on an innate immune system to combat invading pathogens. The system is initiated in the presence of cell wall components from microbes like lipopolysaccharide (LPS), β-1,3-glucan (βG) and peptidoglycan (PG), altogether known as pathogen-associated molecular patterns (PAMPs), via
Xiao-Wen Lin et al.
PloS one, 8(6), e67633-e67633 (2013-06-27)
Toll-like receptors (TLRs) play a pivotal role in the defense against invading pathogens by detecting pathogen-associated molecular patterns (PAMPs). TLR4 recognizes lipopolysaccharides (LPS) in the cell walls of Gram-negative bacteria, resulting in the induction and secretion of proinflammatory cytokines such
Oscar Pizano-Martínez et al.
World journal of gastroenterology, 17(33), 3830-3835 (2011-10-12)
To demonstrate that CD14⁺ cells are an important source of the growth factor YKL-40 in acute and chronic liver damage. Rats were inoculated with one dose of CCl(4) to induce acute damage. Liver biopsies were obtained at 0, 6, 12

Articoli

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Domande

  1. Is there any recommendation in how to store the powder if I will not prepare all the 100 mg, Is it still active to store them in the fridge ?

    1 risposta
    1. Yes, the remaining powder product is stable when stored refrigerated.

      Utile?

Recensioni

Nessuna valutazione

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