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31149

Sigma-Aldrich

Deoxyribonucleic acid, low molecular weight from salmon sperm

Sinonimo/i:

Salmon sperm dsDNA, low molecular weight DNA, DNA

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10 G
55,00 €
50 G
221,00 €

55,00 €


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Cambia visualizzazione
10 G
55,00 €
50 G
221,00 €

About This Item

Numero CAS:
Numero CE:
Numero MDL:
Codice UNSPSC:
41106310
eCl@ss:
32160414
NACRES:
NA.52

55,00 €


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Grado

for molecular biology
puriss. p.a.

Stato

lyophilized powder

Impurezze

≤5% protein

Solubilità

50 mM Tris pH 8.0: 10 mg/mL, clear, faintly brown-yellow

Rapporto di assorbanza

A260/280 nm ~1.4

Temperatura di conservazione

2-8°C

Applicazioni

Various concentrations of Deoxyribonucleic acid, low molecular weight, from salmon sperm has been used:
  • to study DNA binding to silicon-substituted hydroxyapatite nanocrystals.[1]
  • in simple electrochemical DNA biosensors to detect and evaluate DNA damage caused by UV-C radiation and reactive oxygen species.[2]
  • as a precursor in the synthesis of the bio-dots.[3]

Azioni biochim/fisiol

Salmon sperm DNA is often used as a blocking agent invarious research applications. It isknown to improve the specificity and sensitivity of the multi-AP assay byreducing non-specific binding and background signal.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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David Czeiger et al.
American journal of clinical pathology, 135(2), 264-270 (2011-01-14)
Elevated circulating cell-free DNA (CFD) levels were found in patients with cancer. The standard CFD assays are work-intensive and expensive. The aim was to evaluate in patients with cancer a new simple CFD assay. In mice inoculated with cancer cells
P K Ganguli et al.
European journal of biochemistry, 101(2), 377-383 (1979-11-01)
Two native DNAs differing in G + C content were bound equally with the antitumour drug cis-Pt(NH3)2Cl2 at increasing Pt/P ratios. Resulting changes in their ultraviolet absorption spectra show equal fractional decreases in the initially different values of A250/A270 for
Jing Yu et al.
Molecular biology of the cell, 13(2), 558-569 (2002-02-21)
Dramatic changes occur in nuclear organization and function during the critical developmental transition from meiosis to mitosis. The Drosophila nuclear lamina protein YA binds to chromatin and is uniquely required for this transition. In this study, we dissected YA's binding
J J Gao et al.
Journal of immunology (Baltimore, Md. : 1950), 166(11), 6855-6860 (2001-05-22)
LPS is well recognized for its potent capacity to activate mouse macrophages to produce TNF-alpha, an important inflammatory mediator in bacterial infection-related diseases such as septic shock. We demonstrate here that while inducing only low levels of TNF-alpha alone, DNA
Eiji Sunami et al.
Clinical chemistry, 55(3), 559-567 (2009-01-10)
Prostate cancer (PCa) detection using serum-based prostate specific antigen (PSA) is limited by frequent false-positive and -negative results. Genetic aberrations such as allelic imbalance (AI) and epigenetic changes such as promoter hypermethylation have been detected in circulating DNA of cancer

Domande

1–2 di 2 domande  
  1. For the product 31149-50G-F (31149-50G-F), what is the molecular weight, or, the amount of base pairs? Thank you!

    1 risposta
    1. The molecular weight of this product is not determined. This material is produced by mechanical disruption, no chemical or enzymatic treatment has been applied. The estimated molecular weight range of this DNA is 50,000 – 100,000 Da and is primarily double stranded. Note that whole DNA from salmon sperm has a molecular weight of 1.98x10e11 Da. While the number of base pairs(bp) has not been investigated, based on a approximate molecular weight of 650 Da per bp, the estimated chain length range is 77 – 154 bp.

      Utile?

  2. What is the Department of Transportation shipping information for this product?

    1 risposta
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Utile?

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