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S0902

Sigma-Aldrich

SSC Buffer

for Northern and Southern blotting, powder blend

Sinonimo/i:

Saline-sodium citrate buffer

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About This Item

Codice UNSPSC:
41105319
NACRES:
NA.25

Forma fisica

powder blend

pH

6.8-7.2 (20-25 °C, 263 mg/L in water)

Solubilità

water: 263 mg/mL, clear, colorless

Attività estranea

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

Temperatura di conservazione

room temp

Descrizione generale

SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Applicazioni

Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.
Suitable for:
  • Preparing nucleic acids for hybridization
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for Northern and Southern blots

Caratteristiche e vantaggi

  • Multi-purpose use in hybridizations
  • Easily reconstituted and diluted to any concentration needed

Componenti

Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

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Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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