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Documenti fondamentali

D8899

Sigma-Aldrich

Deoxyribonucleic acid, single stranded from calf thymus

lyophilized powder

Sinonimo/i:

DNA single stranded

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About This Item

Numero CAS:
Numero CE:
Numero MDL:
Codice UNSPSC:
41106310
eCl@ss:
32160414
NACRES:
NA.52

Grado

for molecular biology

Livello qualitativo

Stato

lyophilized powder

PM

~50 kb

Composizione

ssDNA, ≥65%

Temperatura di conservazione

−20°C

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Descrizione generale

High quality single-stranded template DNA isolated from the thymus of male and female calves.

Applicazioni

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay and in DNA-binding assay.

Caratteristiche e vantaggi

• Activated
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Quantità

One mg DNA is approx. 25 A260 units.

Nota sulla preparazione

Prepared by a modification of the method of Alberts and Herrick from calf thymus DNA.

Ricostituzione

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves


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J A Hansen et al.
Journal of animal science, 75(7), 1810-1821 (1997-07-01)
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H Kamei
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