Összes fotó(2)
Fontos dokumentumok
T8690
Thioredoxin human
≥90% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), essentially salt-free, lyophilized powder, ≥5 U/mg
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
Javasolt termékek
biológiai forrás
human
Minőségi szint
rekombináns
expressed in E. coli (N-terminal histidine tagged)
Teszt
≥90% (SDS-PAGE)
form
essentially salt-free, lyophilized powder
specifikus aktivitás
≥5 U/mg
molekulatömeg
14 kDa
technika/technikák
activity assay: suitable
oldhatóság
water: soluble 0.50—0.60 mg/mL, clear to slightly hazy, colorless to faint yellow or tan
alkalmasság
suitable for molecular biology
UniProt elérési szám
alkalmazás(ok)
cell analysis
tárolási hőmérséklet
−20°C
Géninformáció
human ... TXN(7295)
Általános leírás
Cloned from Jurkat cell cDNA. Identical to the ADF/Trx (GenBank® Accession Number X77584) and the thioredoxin from human placenta (GenBank® Accession Number AF085844).
Thioredoxin (TXN) is a 12 kDa ubiquitous protein. The gene is located on human chromosome 9q31.3. TXN is mainly located in the cytoplasm.Thioredoxin contains a redox-active dithiol in the active site. Itexists in several forms, the most prevalent ones being the cytosolic (TRX1) andmitochondrial (TRX2) forms.
Alkalmazás
Thioredoxin human can be used for in vitro thioredoxin assay. The product can also be used for assessing Thioredoxin reductase (TRXR) enzyme activity.
Thioredoxin human has been used:
- in mitogen-activated protein kinase kinase 4 (MKK4) in vitro kinase assay
- to study its role in transcription regulation, RNA processing and nuclear pore function in human cells
- in proteomics identification of Trx1 reduction target proteins from the hearts of cardiac specific transgenic (Tg)-Trx1 mice
Biokémiai/fiziológiai hatások
Human Thioredoxin is a recombinant, N-terminal histidine tagged protein. The product induces proliferation in lymphoid cells, fibroblasts as well as in numerous human solid tumor cell lines.The product also stimulates growth in normal as well as leukemic B cell, modifies the transcriptional activity of AP-1and inhibits the apoptosis signal-regulating kinase 1 (ASK1).Thioredoxin (TRX) plays a role in protecting the cells fromelectrophiles, a cytotoxic agent.
Thioredoxin (TXN) has disulfide-reducing activity. It acts as a cytokine to reduce inflammation. TXN functions as a scavenger for reactive oxygen intermediates (ROIs), which are produced in various oxidative stress. Thioredoxin induces cellular proliferation to decrease oxidative stress. TXN is a prognostic biomarker for various cancers, such as lung, pancreatic and hepatocellular carcinoma. It contributes to protein reduction, tissue development and redox regulation.
Mediates the reduction of disulfide bonds in proteins.
Egység definíció
One unit will cause a ΔA650 of 1.0 in 1 min at 25 °C in the insulin reduction assay.
Jogi információk
GenBank is a registered trademark of United States Department of Health and Human Services
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
Eyeshields, Gloves, type N95 (US)
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az ügyfelek ezeket is megtekintették
Cexiong Fu et al.
Molecular & cellular proteomics : MCP, 8(7), 1674-1687 (2009-05-07)
Thioredoxin 1 (Trx1) is a key redox modulator that is functionally conserved across a wide range of species, including plants, bacteria, and mammals. Using a conserved CXXC motif, Trx1 catalyzes the reduction of cysteine disulfides and S-nitrosothiols. In contrast to
TXN (thioredoxin)
Chen Z, et al.
Atlas of Genetics and Cytogenetics in Oncology and Haematology (2010)
Elucidation of thioredoxin target protein networks in mouse
Fu C, et al.
Molecular and Cellular Proteomics, 8(7), 1674-1687 (2009)
Christopher J Boehler et al.
PloS one, 8(8), e71525-e71525 (2013-08-13)
Thioredoxin reductase-1 (TRXR-1) is the sole selenoprotein in C. elegans, and selenite is a substrate for thioredoxin reductase, so TRXR-1 may play a role in metabolism of selenium (Se) to toxic forms. To study the role of TRXR in Se
Shaoxiong Wang et al.
Journal of the American Heart Association, 8(8), e012192-e012192 (2019-04-09)
Background Dopamine D5 receptor (D5R) plays an important role in the maintenance of blood pressure by regulating renal sodium transport. Our previous study found that human D5R mutant F173L transgenic ( hD 5 R F173L-TG) mice are hypertensive. In the
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