CHO-K1 Cell Line from hamster

85051005, hamster ovary, Epithelial

• Szinonimák: CHO cell clone K1 Cells, CHOK1 Cells
• UNSPSC kód: 41106514

Tulajdonságok

• product name: CHO-K1 Cell Line from hamster, 85051005, from hamster ovary
• biológiai forrás: hamster ovary
• növekedési mód: Adherent
• kariotípus: Hypodiploid, modal no. 20
• morfológia: Epithelial
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Hamster Chinese ovary

Sejtvonal leírása

A subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline due to the absence of the gene for proline synthesis, the block in the biosynthetic chain lies in the step converting glutamic acid to glutamine gamma serialdehyde. They undergo morphological changes in response to cholera toxin.

Alkalmazás

CHO-K1 cell line from hamster has been used to study the effect of plasmid pCM01 induction on the association of bacteria with CHO-K1 cells by the gentamicin protection assay. It has also been cultured with or without lactate to measure time-series for cell proliferation.

Nutritional and gene expression studies

Táptalaj

Ham′s F12+ 2mM Glutamine+ 10% Foetal Bovine Serum FBS / FCS.

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25%Trypsin or Trypsin/EDTA; 5% CO₂; 37°C.

Egyéb megjegyzések

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