S0389

Sucrose

for molecular biology, ≥99.5% (GC)

• Szinonimák: α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopyranosyl β-D-fructofuranoside, β-D-Fructofuranosyl-α-D-glucopyranoside, D(+)-Saccharose, Sugar
• Tapasztalati képlet (Hill-képlet): C₁₂H₂₂O₁₁
• CAS-szám: 57-50-1
• Molekulatömeg: 342.30
• Beilstein: 90825
• EC-szám: 200-334-9
• MDL-szám: MFCD00006626
• UNSPSC kód: 12352201
• PubChem Substance ID: 24899423
• NACRES: NA.31

Tulajdonságok

• biológiai forrás: sugar cane
• Minőségi szint: 300
• grade: for molecular biology
• Teszt: ≥99.5% (GC)
• form: crystals
• technika/technikák: HPLC: suitable; cryopreservation: suitable; electron microscopy: suitable; enzyme immunoassay: suitable; gas chromatography (GC): suitable; immunohistochemistry: suitable
• szennyeződések: ≤0.1% free glucose
• szín: white
• hasznos pH-tartomány: 5.5-7 (25 °C, 342 g/L)
• mp: 185-187 °C (lit.)
• oldhatóság: H₂O: soluble 50 g + 50 mL
• kation nyomok: heavy metals (as Pb): ≤5 ppm
• absorption: ≤0.15 at 280 at 50%; ≤0.20 at 260 at 50%
• alkalmasság: suitable for molecular biology
• alkalmazás(ok): agriculture; agriculture; life science and biopharma; metabolomics; sample preservation
• idegen aktivitás: DNase, RNase, protease, none detected
• tárolási hőmérséklet: room temp
• SMILES string: OC[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C12H22O11/c13-1-4-6(16)8(18)9(19)11(21-4)23-12(3-15)10(20)7(17)5(2-14)22-12/h4-11,13-20H,1-3H2/t4-,5-,6-,7-,8+,9-,10+,11-,12+/m1/s1
• Nemzetközi kémiai azonosító kulcs: CZMRCDWAGMRECN-UGDNZRGBSA-N

Leírás

Általános leírás

Sucrose, a disaccharide composed of glucose and fructose, serves as an energy source in various biological processes. In cell biology, sucrose acts as a cryoprotectant, preserving cell viability during freezing and thawing. It is also added as a supplement in culture media, aiding in maintaining osmotic balance and enhancing cell survival.In molecular biology, sucrose is crucial in gradient solutions for DNA and RNA purification and in density centrifugation for organelle separation.

Alkalmazás

Sucrose has been used:

• to perform cryoprotection for kidney tissues
• as a component in the solid germination medium to germinate pollen grains
• in the triphenyl-tetrazolium chloride (TTC) test to check the viability of pollen grains
• in the cryoprotection of fixed brain tissues for immunohistochemistry

Biokémiai/fiziológiai hatások

Sucrose is used as a transport and storage molecule in most plants due to its limited chemical reactivity. It can be used to prepare density gradients for the separation of cell/organelle. Sucrose can be used in the plant, insect, and bacterial culture media as a supplement. In addition, sucrose can also be used in several enzymatic assays.

Tulajdonságok és előnyök

• Ideal for Molecular Biology, Cell Biology and Biochemical research
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Tested for DNase, RNase, and proteases
• Molecular biology grade sucrose, sourced from sugar cane

Egyéb megjegyzések

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Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)