P6248

Anti-Parkin antibody, Mouse monoclonal

clone PRK8, purified from hybridoma cell culture

• Szinonimák: Anti-AR-JP, Anti-LPRS2, Anti-PARK2, Anti-PDJ
• MDL-szám: MFCD02263896
• UNSPSC kód: 12352203
• NACRES: NA.41
• konjugátum: unconjugated
• application: ARR; WB
• klón: PRK8, monoclonal
• faj reaktivitás: mouse, rat, hamster, human
• citations: 22
• technika/technikák: microarray: suitable; western blot: 0.25-0.5 μg/mL using using rat brain cytosolic S1 extract

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 200
• konjugátum: unconjugated
• antitest forma: purified immunoglobulin
• antitest terméktípus: primary antibodies
• klón: PRK8, monoclonal
• Forma: buffered aqueous solution
• faj reaktivitás: mouse, rat, hamster, human
• koncentráció: ~2 mg/mL
• technika/technikák: microarray: suitable; western blot: 0.25-0.5 μg/mL using using rat brain cytosolic S1 extract
• izotípus: IgG2b
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... PARK2(5071) ; mouse ... Park2(50873) ; rat ... Park2(56816)

Leírás

Általános leírás

Monoclonal Anti- Parkin (mouse IgG2b isotype) is derived from the hybridoma PRK8 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human Parkin. Parkin is an ubiquitin-protein ligase expresses in many tissues such as brain, testis, skeletal muscle and heart. The parkin gene encodes a protein of 465 amino acid residues with moderate similarity to ubiquitin at the amino-terminus and a ring in between ring fingers (IBR)-ring-finger motif at the carboxyl-terminus. The gene has 12 exons.

Parkin is an ubiquitin-protein ligase expresses in many tissues such as brain, testis, skeletal muscle and heart. It is a RING-type E3 ubiquitin-protein ligase that facilitates the ubiquitination of misfolded proteins and protects from neurotoxic effect caused by these proteins. Monoclonal anti-parkin antibody can be used in microarray and immunoprecipitation assays. It can also be used in western blotting. Mouse anti-parkin antibody reacts specifically with parkin (50kD approx) of rat, hamster, mouse and human.

Immunogén

recombinant human Parkin.

Alkalmazás

Monoclonal anti-parkin antibody has been used in:

• Enzyme linked immunosorbent assay (ELISA)
• Immunoblotting
• Immunoprecipitation[63}
• Immunocytochemistry
• to label parkin in pull-down assay

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)