Összes fotó(2)
Fontos dokumentumok
P0068
Anti-p62/SQSTM1 (C-terminal) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Szinonimák:
Anti-Sequestosome 1, Anti-Ubiquitin-binding protein p62
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
UNSPSC kód:
12352203
NACRES:
NA.41
konjugátum:
unconjugated
application:
IP
WB
WB
klón:
polyclonal
faj reaktivitás:
rat, mouse, human
citations:
37
technika/technikák:
immunoprecipitation (IP): 2-4 μg using whole extract of NIH-3T3 cells
western blot: 2-4 μg/mL using whole extracts of rat PC12 cells
western blot: 4-8 μg/mL using whole extracts of human A549 cells.
western blot: 2-4 μg/mL using whole extracts of rat PC12 cells
western blot: 4-8 μg/mL using whole extracts of human A549 cells.
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
konjugátum
unconjugated
antitest forma
affinity isolated antibody
antitest terméktípus
primary antibodies
klón
polyclonal
Forma
buffered aqueous solution
molekulatömeg
antigen ~62 kDa
faj reaktivitás
rat, mouse, human
koncentráció
~1.0 mg/mL
technika/technikák
immunoprecipitation (IP): 2-4 μg using whole extract of NIH-3T3 cells
western blot: 2-4 μg/mL using whole extracts of rat PC12 cells
western blot: 4-8 μg/mL using whole extracts of human A549 cells.
UniProt elérési szám
kiszállítva
dry ice
tárolási hőmérséklet
−20°C
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... SQSTM1(8878)
mouse ... Sqstm1(18412)
rat ... Sqstm1(113894)
Általános leírás
The p62 protein/sequestosome 1 (SQSTM1) comprises a Phox and Bem1p (PB1) domain at the NH2-terminal, a ZZ type zinc finger domain, a Pro, Glu, Ser, and Thr (PEST) region consisting of putative phosphorylation sites, and a ubiquitin-associated (UBA) domain at the COOH-terminal. The SQSTM1 gene is mapped to the human chromosome location 5q35.3.
Egyediség
Anti-p62/SQSTM1 (C-terminal) recognizes human, rat, and mouse p62/SQSTM1.
Alkalmazás
Rabbit polyclonal anti-SQSTM1 antibody has been used:
- in immunoblotting
- in immunoprecipitation
- as a probe to determine the presence and roles of p62 protein/sequestosome 1 in processes such as autophagy
Biokémiai/fiziológiai hatások
The p62 protein/sequestosome 1 (SQSTM1) is a multifunctional protein, which binds to ubiquitin. It acts as a scaffold protein for the nuclear factor kappa-B (NF-ΚB) signaling pathway. SQSTM1 also regulates apoptosis and autophagy. Mutations in this gene lead to sporadic and familial Paget disease of bone. It is also associated with protein aggregation diseases, such as Lewy bodies in Parkinson′s disease, neurofibrillary tangles in Alzheimer′s disease, and huntingtin aggregates. SQSTM1 is commonly found in inclusion bodies containing polyubiquitinated protein aggregates that accumulate in several degenerative diseases. p62 polymerizes through its N-terminal Phox and Bem1p (PB1) domain and interacts with polyubiquitinated proteins through its C-terminal ubiquitin-associated (UBA) domain. p62 acts as a linker between protein aggregates and the autophagy machinery.
Fizikai forma
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Tárolás és stabilitás
For continuous use, store at 2–8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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kapcsolódó termék
Tárolási osztály kódja
10 - Combustible liquids
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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