NUC201

Nuclei Isolation Kit: Nuclei PURE Prep

sufficient for 15 nuclei preparations (~1-10×10⁷ cells or 1g of tissue per preparation)

• Szinonimák: Sucrose centrifugation nuclei isolation
• UNSPSC kód: 12352207
• NACRES: NA.32

Tulajdonságok

• használat: sufficient for 15 nuclei preparations (~1-10×10⁷ cells or 1g of tissue per preparation)
• Minőségi szint: 200
• kiszerelés: pkg of 1 kit
• tárolási körülmény: dry at room temperature
• alkalmazás(ok): cell analysis
• idegen aktivitás: nuclease and protease, free
• kiszállítva: wet ice
• tárolási hőmérséklet: 2-8°C

Leírás

Alkalmazás

For preparation of pure nuclei and fragile nuclei from cell lines and solid tissues.

Biokémiai/fiziológiai hatások

The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. The sucrose concentration that is suitable for a particular cell type is determined empirically by the user. The sucrose concentrate and sucrose cushion buffer give the user flexibility to modify the density of the sucrose cushion as appropriate. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

Kit Components Only

• Nuclei PURE Lysis Buffer 180 mL

Biztonsági adatok

• Piktogramok: GHS05,GHS09
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H315,H318,H410
• Óvintézkedésre vonatkozó mondatok: P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Veszélyességi osztályok: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
• Tárolási osztály kódja: 10 - Combustible liquids
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable