N8786

Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal

clone 414, purified from hybridoma cell culture

• MDL-szám: MFCD02263844
• UNSPSC kód: 12352203
• NACRES: NA.41
• konjugátum: unconjugated
• application: EM; ICC; IP; WB
• klón: 414, monoclonal
• faj reaktivitás: mouse, rat, yeast, Xenopus, human
• citations: 10
• technika/technikák: electron microscopy: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 2-4 μg/mL using HeLa total cell extract

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 200
• konjugátum: unconjugated
• antitest forma: purified immunoglobulin
• antitest terméktípus: primary antibodies
• klón: 414, monoclonal
• Forma: buffered aqueous solution
• molekulatömeg: antigen 62-210 kDa
• faj reaktivitás: mouse, rat, yeast, Xenopus, human
• koncentráció: ~3 mg/mL
• technika/technikák: electron microscopy: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 2-4 μg/mL using HeLa total cell extract
• izotípus: IgG1
• kiszállítva: dry ice
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... NPIPB15(440348)

Leírás

Általános leírás

Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma 414 produced by the fusion of mouse myeloma cells (NS-1 cells) and splenocytes from BALB/c mice immunized with rat liver nuclei extract. The nuclear pore complexes (NPCs) are structurally highly conserved in all eukaryotic cells. They contain 30-40 different proteins resulting in a complex with a molecular weight of ~50 kDa.

Nuclear pore complexes (NPCs) are aqueous channels that impart access to the nucleus and modulate the transport of RNA, proteins and ribosome subunits across the nuclear envelope. Nucleoporins, which are conserved proteins, form a complex network and generate these channels. Each NPC is a large, complex cylindrical structure consisting of around 500 nucleoporins.

Immunogén

rat liver nuclei extract.

Alkalmazás

Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal has been used for immunohistochemistry.

Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal has been used in:

• immunoblotting
• immunoprecipitation
• immunocytochemistry
• immunoelectron microscopy
• immunofluorescence

Biokémiai/fiziológiai hatások

Nucleocytoplasmic exchanges occur through nuclear pore complexes (NPCs) that are circular apertures in the nuclear envelope where the inner and the outer nuclear membrane are joined. Through the NPC there is a passive transport of small molecules (ions and water) that are smaller than 9-10 nm. Large molecules such as RNA and proteins have to be actively transported through the NPC in an energy and signal dependent manner. Many proteins interact with members of the NPC complex.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: nwg
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable