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MAK062

Sigma-Aldrich

IDH Activity Assay Kit

sufficient for 100 colorimetric tests

Szinonimák:

Isocitrate Dehydrogenase Activity Assay

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(3)

About This Item

UNSPSC kód:
12161503
NACRES:
NA.84

használat

sufficient for 100 colorimetric tests

észlelési módszer

colorimetric

vonatkozó betegség(ek)

cancer

tárolási hőmérséklet

−20°C

Géninformáció

human ... IDH1(3417) , IDH2(3418) , IDH3B(3420) , IDH3G(3421)
mouse ... IDH1(15926) , IDH2(269951) , IDH3B(170718) , IDH3G(15929)
rat ... IDH1(24479) , IDH2(361596) , IDH3B(94173) , IDH3G(25179)

Általános leírás

Isocitrate dehydrogenase (IDH) catalyzes the conversion of isocitrate to α-ketoglutarate. In eukaryotes, there are three isozymes of IDH, the mitochondrial IDH2 and IDH3, and the cytoplasmic/ peroxisomal IDH1. All three IDH family members require the presence of a divalent cation (Mg2+ or Mn2+) and either the electron-accepting cofactor NADP+ (IDH1 and IDH2) or NAD+ (IDH3) for their enzymatic activity. IDH1 and IDH2 mutations resulting in neomorphic enzymatic activity are found in certain cancers such as glioblastoma, acute myeloid leukemia, and colon cancer. This neoactivity shows a change in the substrate specificity resulting in the conversion of α-ketoglutarate to 2-hydroxyglutarate. Mutations in IDH family members are also associated with Ollier disease and Maffucci syndrome.

Alkalmazás

IDH Activity Assay Kit has been used to determine the activity of isocitrate dehydrogenase in samples.

Tulajdonságok és előnyök

Compatible with high-throughput handling systems.

Alkalmasság

Suitable for the measurement of isocitrate dehydrogenase (IDH) (NAD+ - dependent, NADP+ -dependent or both IDHs) activity in biological samples including tissue, cells and serum

Elv

The Isocitrate Dehydrogenase Activity Assay kit provides a simple and direct procedure for measuring NADP+-dependent, NAD+-dependent, or both NADP+ and NAD+-dependent IDH activity in a variety of samples. IDH activity is determined using isocitrate as the substrate in an enzyme reaction, which results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of IDH is the amount of enzyme that will generate 1.0 μmole of NADH or NADP per minute at pH 8.0 at 37 °C.

Alkalmazás

Product No.
Leírás
Árazás

kapcsolódó termék

Product No.
Leírás
Árazás

06693

Timestrip Plus™ -20 °C

Piktogramok

Health hazardCorrosion
GHS08,GHS05

Figyelmeztetés

Danger

Figyelmeztető mondatok

H314,H334

Veszélyességi osztályok

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Tárolási osztály kódja

8A - Combustible corrosive hazardous materials

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
8M12K07560
8F20K07560
7L10K07560
7J08K07560
7G15K07560

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COA keresése

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D-2-Hydroxyglutarate (D2HG) Assay Kit sufficient for 200 fluorometric reactions

Sigma-Aldrich

MAK320

D-2-Hydroxyglutarate (D2HG) Assay Kit

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Protocols

Chiral LC-MS/MS of D- and L-2-Hydroxyglutaric Acid Biomarkers

We describe here a rapid and sensitive method to separate and measure D-2-OHG and L-2-OHG enantiomers using high-resolution mass spectrometry (HRMS) detection.

Chromatograms

application for HPLC

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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