Ugrás a tartalomra
Merck

I4144

Sigma-Aldrich

IL-3 mouse

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Szinonimák:

IL-3, mIL-3, Interleukin-3 from mouse

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

MDL-szám:
UNSPSC kód:
12352202
NACRES:
NA.77

Terméknév

Interleukin-3 from mouse, IL-3, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

biológiai forrás

mouse

Minőségi szint

rekombináns

expressed in E. coli

Teszt

≥98% (SDS-PAGE)

Forma

lyophilized powder

hatékonyság

0.01-0.1 ng/mL ED50/EC50

minőség

endotoxin tested

molekulatömeg

15.1 kDa

kiszerelés

pkg of 10 and 50 μg

technika/technikák

cell culture | mammalian: suitable

szennyeződések

≤1.000 EU/μg

szín

white to yellow

UniProt elérési szám

tárolási hőmérséklet

−20°C

Géninformáció

mouse ... Il3(16187)

Általános leírás

Interleukin-3 is a hematopoietic growth factor, which stimulates basophil development and triggers histamine, IL-4 and IL-6 release. It improves monocyte and macrophage activation and phagocytosis, enhances the secretory function of eosinophils. Interleukin-3 promotes endothelial cell proliferation and migration.

Alkalmazás

Interleukin-3 from mouse has been used for the determination of 5-lipoxygenase product leukotriene C4 (LTC4) formation. It has also been used as a component of RPMI 1640 medium for cell transfection.

Biokémiai/fiziológiai hatások

Interleukin-3 (IL-3) is a multifunctional protein, originally called colony forming unit-stimulating activity (CFU-SA), and is produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 has been shown to induce colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar-suspended bone marrow cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and to induce IgG secretion from activated B cells. Other synonyms for IL-3 include pan-specific hemopoietin, multicolony stimulating factor, mast cell growth factor, burst promoting activity, histamine-producing cell stimulating factor, P cell stimulating factor, and WEHI-3 factor.

Fizikai forma

Lyophilized with 0.5 mg of bovine serum albumin. Sterile filtered through a 0.2 μm filter.

Analízis megjegyzés

The biological activity is measured by the dose-dependent stimulation of the proliferation of mouse M-NFS-60 cells.

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, type N95 (US)


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Analitikai tanúsítványok (COA)

Lot/Batch Number

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Dokumentumtár megtekintése

Michèle Wiederkehr-Adam et al.
The Journal of biological chemistry, 278(18), 16117-16128 (2003-02-20)
Signal transducers and activators of transcription (STAT) 1 and STAT3 are activated by overlapping but distinct sets of cytokines. STATs are recruited to the different cytokine receptors through their Src homology (SH) 2 domains that make highly specific interactions with
Synthesis and evaluation of benzoxazole derivatives as 5-lipoxygenase inhibitors
Song H, et al.
Bioorganic & Medicinal Chemistry, 18(21), 7580-7585 (2010)
Characterization of phosphopeptide motifs specific for the Src homology 2 domains of signal transducer and activator of transcription 1 (STAT1) and STAT3
Wiederkehr-Adam M, et al.
Test, 278(18), 16117-16128 (2003)
Interleukin-3-deficient mice have increased resistance to blood-stage malaria.
Auclair SR et al
Infection and Immunity, 82(3), 1308-1314 (2014)
Serena De Vita et al.
PloS one, 9(5), e96209-e96209 (2014-05-03)
Systemic Mastocytosis (SM) is a clonal disease characterized by abnormal accumulation of mast cells in multiple organs. Clinical presentations of the disease vary widely from indolent to aggressive forms, and to the exceedingly rare mast cell leukemia. Current treatment of

Cikkek

Interleukins, released from leukocytes, include IL-1β playing crucial roles in inflammation, immune responses, and anti-tumor immunity.

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