GE28-9365-51

Hisprep^™ Fast Flow 16/10

Cytiva 28-9365-51

• Szinonimák: 16/10 Hisprep, Hisprep Column, Hisprep Fast Flow Column
• UNSPSC kód: 23151817
• NACRES: NA.56

Tulajdonságok

• kiszerelés: pkg of 1 ea
• gyártó/kereskedő neve: Cytiva 28-9365-51
• parameter: 1.5 bar (22 psi) (Over the Packed Bed During Operation); 22 psi
• ágyméret: 16 mm × 100 mm
• ágytérfogat: 20 mL
• oszlop belső átmérő: 16 mm
• matrix: 6% cross-linked agarose
• részecskeméret: 45-165 μm
• átlagos átmérő: 90 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• working range: 3-12(Ni²⁺-stripped medium.)
• kapacitás: ~40 mg binding capacity(histidine-tagged protein)
• alkalmasság: suitable for bioprocess medium

Leírás

Általános leírás

HisPrep^™ FF 16/10 is a ready-to-use column, prepacked with precharged Ni Sepharose^™ 6 Fast Flow. The column is an excellent choice for preparative, scale-up purification of histidine-tagged recombinant proteins.

HisPrep^™ FF 16/10 columns are prepacked with Ni Sepharose^™ 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose^™ 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni²⁺ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

Tulajdonságok és előnyök

• Optimized for convenient scale-up purification of histidine-tagged proteins.
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
• Negligible leakage of Ni²⁺.
• High binding capacity, approx. 40 mg/mL medium.
• Convenient and time-saving 20 mL prepacked HiPrep^™ format.

Tárolás és stabilitás

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analízis megjegyzés

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Egyéb megjegyzések

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Jogi információk

HiPrep is a trademark of Cytiva

HisPrep is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Biztonsági adatok

• Piktogramok: GHS09
• Figyelmeztetés: Warning
• Figyelmeztető mondatok: H226
• Óvintézkedésre vonatkozó mondatok: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Tárolási osztály kódja: 3 - Flammable liquids