G3272

Guanidine hydrochloride

for molecular biology, ≥99%

• Szinonimák: Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride
• Lineáris képlet: NH₂C(=NH)NH₂ · HCl
• CAS-szám: 50-01-1
• Molekulatömeg: 95.53
• Beilstein: 3591990
• EC-szám: 200-002-3
• MDL-szám: MFCD00013026
• UNSPSC kód: 12352107
• PubChem Substance ID: 24895119
• NACRES: NA.31

Tulajdonságok

• biológiai forrás: synthetic (organic)
• Minőségi szint: 400
• grade: for molecular biology
• Teszt: ≥99%
• Forma: crystalline powder
• tárolási körülmény: (Tightly closed. Dry. )
• technika/technikák: RNA extraction: suitable
• szennyeződések: ≤0.3% water (Karl Fischer)
• szín: white
• pH: (25 °C, 4.6 - 6/573 g/L)
• mp: 180-185 °C (lit.)
• oldhatóság: H₂O: 6 M, clear, colorless
• sűrűség: 1.3 g/cm³ (lit.)
• kation nyomok: Pb: ≤5 ppm
• UV-elnyelés: λ: 260 nm A_max: ≤0.10; λ: 290 nm A_max: ≤0.05
• idegen aktivitás: DNase, RNase, none detected
• SMILES string: Cl[H].NC(N)=N
• InChI: 1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H
• Nemzetközi kémiai azonosító kulcs: PJJJBBJSCAKJQF-UHFFFAOYSA-N
• Géninformáció: human ... KCNA1(3736) , KCNA10(3744) , KCNA2(3737) , KCNA3(3738) , KCNA4(3739) , KCNA5(3741) , KCNA6(3742) , KCNA7(3743) , KCNB1(3745) , KCNB2(9312) , KCNC1(3746) , KCNC2(3747) , KCNC3(3748) , KCNC4(3749) , KCND1(3750) , KCND2(3751) , KCND3(3752) , KCNF1(3754) , KCNG1(3755) , KCNG2(26251) , KCNG3(170850) , KCNG4(93107) , KCNH1(3756) , KCNH2(3757) , KCNH3(23416) , KCNH4(23415) , KCNH5(27133) , KCNH6(81033) , KCNH7(90134) , KCNH8(131096) , KCNQ1(3784) , KCNQ2(3785) , KCNQ3(3786) , KCNQ4(9132) , KCNQ5(56479) , KCNS1(3787) , KCNS2(3788) , KCNS3(3790) , KCNV1(27012) , KCNV2(169522)

Leírás

Általános leírás

Guanidine hydrochloride, also known as aminoformamidine hydrochloride, is a versatile compound widely used in molecular biology and biochemical research. Functioning as a potent chaotropic agent, it disrupts protein and nucleic acid structures, making it invaluable for purifying proteins, particularly mRNA. Its denaturing capabilities extend to unfolding proteins, aiding their purification and subsequent refolding, and it can even solubilize denatured insoluble proteins at higher concentrations.

At lower concentrations, guanidine hydrochloride exhibits a unique effect, promoting the refolding of denatured proteins and restoring enzymatic activity. This property is advantageous for protein renaturation studies. In RNA extraction, guanidine hydrochloride acts as a robust denaturant, disrupting cell structures and inactivating RNA enzymes, ensuring the integrity of extracted RNA. In summary, guanidine hydrochloride is a versatile compound with applications in protein purification, nucleic acid isolation, and protein refolding studies, showcasing its multifaceted utility in molecular biology and biochemical research.

Alkalmazás

Guanidine hydrochloride has been used:

• as a component of the extraction buffer for the extraction of proteoglycans
• in extraction during protein fractionation of ATDC5 cell lines
• as a chemical additive to study its effective absorbance spectra in structural analysis
• to incubate the device chip to release prostate-specific antigen and for regeneration of aptamer
• as a component of the 2,4-dinitrophenylhydrazine (DNPH) solution for detecting protein carbonyls by protein carbonylation study
• in RNA isolation to dissociate nucleoproteins and inhibit RNase

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Biokémiai/fiziológiai hatások

Guanidine hydrochloride (GuHCl) is a small hydroscopic molecule. It plays a role in inhibiting heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) activity in vivo. It is a potent denaturant and inactivator of several enzymes and proteins. GuHCl can inactivate aminoacylase and papain. It is involved in unfolding and inactivation of alkaline phosphatase in Haliotis diversicolor. At higher concentrations, GuHCl is involved in virus inactivation such as Herpes simplex virus 1 (HSV-1).

Tulajdonságok és előnyök

• Highly versatile surfactant for your cell biology and biochemical research
• Suitable for sensitive molecular biology applications
• Free from Exonuclease, Dnase and Rnase
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Vigyázat

May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical.

Elkészítési megjegyzés

In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes.
The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.

Egyéb megjegyzések

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Biztonsági adatok

• Piktogramok: GHS07
• Figyelmeztetés: Warning
• Figyelmeztető mondatok: H302 + H332,H315,H319
• Óvintézkedésre vonatkozó mondatok: P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Veszélyességi osztályok: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: dust mask type N95 (US), Eyeshields, Faceshields, Gloves