HOM-2 human cell line

IHW Number: 9005, 98092902

• UNSPSC kód: 41106514

Tulajdonságok

• biológiai forrás: human blood
• etnikum: Canadian
• nem: female
• növekedési mód: Suspension
• kariotípus: Not specified
• morfológia: Lymphoblastoid
• technika/technikák: cell culture | mammalian: suitable
• kiszállítva: dry ice

Leírás

Általános leírás

Consanguineous: No

Homozygous: Yes

Sejtvonal leírása

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNS profil

A: 301
Cw: 102
B: 27052
HLA DRA: 101
HLA DRB1: 101
HLA DRB6: 101
HLA DQA1: 101
HLA DQB1: 501
HLA DPA1: 1
HLA DPB1: 401

Szerológiai profil

HLA A: 3
HLA A ief: 3.2
HLA C: 1
HLA B: 27
HLA B ief: 27.5
Bw4/bw6: 4
HLA Dw: 1
HLA DR: 1
HLA DQ: 5

Táptalaj

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Szubkultúra rutin

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Egyéb megjegyzések

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Jogi nyilatkozat

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.