A375 Cell Line human

88113005, human skin, Epithelial

• Szinonimák: A-375 Cells, A375-MEL Cells
• UNSPSC kód: 41106514

Tulajdonságok

• product name: A375 Cell Line human, 88113005
• biológiai forrás: human skin
• leírás: Human malignant melanoma
• növekedési mód: Adherent
• kariotípus: Modal no. = 62 (hypotriploid) 9 marker chromosomes. Normal N2, N6 and N22 present.
• morfológia: Epithelial
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• vonatkozó betegség(ek): cancer
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Human malignant melanoma

Sejtvonal leírása

Derived from a 54 year old female with malignant melanoma. The cell line produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice.

Alkalmazás

A375 cell line has been used to obtain paracrine factors for prolonged culture of mesenchymal stromal cells (MSCs). It has also been used to study oncolytic activity of the peptide LTX-315.

Tumourigenicity studies

Táptalaj

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Egyéb megjegyzések

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