CALU 1

93120818, human lung, Epithelial

• Szinonimák: Calu1 Cells
• UNSPSC kód: 41106514
• biológiai forrás: human lung
• növekedési mód: Adherent
• kariotípus: Hypotriploid, modal no. 62. No Y chromosome.
• morfológia: Epithelial
• termékek: Not specified
• receptorok: Not specified
• vonatkozó betegség(ek): cancer

Tulajdonságok

• Terméknév: CALU 1, 93120818
• biológiai forrás: human lung
• növekedési mód: Adherent
• kariotípus: Hypotriploid, modal no. 62. No Y chromosome.
• morfológia: Epithelial
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• vonatkozó betegség(ek): cancer
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Human Caucasian lung epidermoid carcinoma

Sejtvonal leírása

Derived from a 47 year old Caucasian male with an epidermoid carcinoma of the lung (grade III). The line will form epidermoid carcinomas in nude mice. Tumours also develop in steroid treated hamsters. Ultrastructure consists of numerous microvilli, prominent rough endoplasmic reticulum, lysosomes, lipid inclusions and no virus particles. The HLA profile is: A-10,11 B15, w35. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Alkalmazás

Tumourigenicity studies

DNS profil

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 11
D5S818: 10,12
D7S820: 9,10
THO1: 9.3
TPOX: 8
vWA: 15,16

Táptalaj

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Egyéb megjegyzések

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