PC-9 (formerly known as PC-14)

90071810, human lung, Heterogeneous mixture of round cells and spindle shaped cells

• Szinonimák: PC-9/S1 Cells, PC-9S1 Cells, PC9 Cells
• UNSPSC kód: 41106514

Tulajdonságok

• Terméknév: PC-9 (formerly known as PC-14), 90071810
• biológiai forrás: human lung
• növekedési mód: Adherent/suspension
• kariotípus: Not specified
• morfológia: Heterogeneous mixture of round cells and spindle shaped cells
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• vonatkozó betegség(ek): cancer
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Human adenocarcinoma

Sejtvonal leírása

Derived from a human adenocarcinoma from lung tissue which remains undifferentiated.

PC-14 was originally deposited with the Riken BioResource Center as a cell line derived from human lung adenocarcinoma (undifferentiated type) in 1989. The RIKEN BioResource Center subsequently informed the European Collection of Cell Cultures (ECACC) that short tandem repeat (STR) DNA profile analysis, undertaken in collaboration Immuno-Biological Laboratories Co., Ltd Japan, has shown this cell line to be identical to PC-9 a cell line derived from human lung adenocarcinoma (differentiated type). The misidentification occurred prior to the cell line being deposited at the RIKEN BioResource Center. The name of the cell line has been changed to PC-9 to reflect this finding.

Alkalmazás

PC-9 cell line has been used to study the effects of α-tocopherol addition on various cytotoxic drugs.

Tumourigenicity studies

DNS profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8
D16S539: 9
D5S818: 11
D7S820: 10
THO1: 7
TPOX: 11
vWA: 17

Táptalaj

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Szubkultúra rutin

Seed cells at 2-4 x 10,000 cells/cm²; 5% CO₂; 37°C. Remove attached cells by brief treatment with 0.25% trypsin or trypsin/EDTA.  

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