HeLa Cell Line human

93021013, human cervix, Epithelial

• Szinonimák: HeLa Cells, HeLa-CCL2 Cells, Henrietta Lacks Cells
• UNSPSC kód: 41106514

Tulajdonságok

• product name: HeLa Cell Line human, epitheloid cervix carcinoma
• biológiai forrás: human cervix
• növekedési mód: Adherent
• kariotípus: aneuploidy
• morfológia: Epithelial
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• vonatkozó betegség(ek): cancer
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Human cervix epitheloid carcinoma

Sejtvonal leírása

Derived from a cervical carcinoma from a 31 year old female. This was the first aneuploid line derived from human tissue maintained in continuous cell culture. Susceptible to Poliovirus type I and adenovirus type 3. Identified as a contaminant in many other cell lines. The cells should be handled under laboratory containment level 2. Ethnicity: Black.

Alkalmazás

HeLa cell line has been used to study whether Zika virus (ZIKV) infection alters centrosome number and spindle positioning in neural progenitor cells. It has also been used to evaluate the cytotoxicity of extracts, fruits and jams from Sorbus sps.

Virus studies

DNS profil

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 12,14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

Táptalaj

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino AcidsNEAA + 10% Foetal Bovine Serum FBS / FCS.

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1.3x10,000 cells/cm² using 0.25% Trypsin/EDTA; 5% CO₂; 37°C.

Egyéb megjegyzések

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.