CHO Cell Line from Chinese hamster ovary

85050302, hamster ovary, Epithelial

• Szinonimák: CHO Cells, CHO-ori Cells, Chinese Hamster Ovary Cells, Chinese hamster ovary cell line
• UNSPSC kód: 41106514

Tulajdonságok

• product name: CHO Cell Line from Chinese hamster ovary, 85050302, from hamster ovary
• biológiai forrás: hamster ovary
• elérhetőség: not available in Japan
• növekedési mód: Adherent
• kariotípus: Hypodiploid, modal no. 20
• morfológia: Epithelial
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Hamster Chinese ovary

Sejtvonal leírása

A subclone of the parental CHO cell line originated by Puck in 1957. The cells have an absolute requirement for L-proline.

Alkalmazás

CHO Cell Line from Chinese hamster ovary has been used:

• to generate CHO cells expressing LY6/PLAUR domain containing 3 (LYPD3)
• to carry out the vaginolysin (VLY) cytolytic activity assay
• as host cells to produce glucagon-like peptide 1 receptor (GLP1R)

Nutritional and gene expression studies

Táptalaj

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Egyéb megjegyzések

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.