CHO Protein-Free Cell Line from Chinese hamster ovary cells

102307, hamster ovary, Suspension

• UNSPSC kód: 41106514
• biológiai forrás: hamster ovary
• növekedési mód: Suspension
• kariotípus: Not specified
• morfológia: Suspension
• termékek: Not specified
• receptorok: Not specified

Tulajdonságok

• Terméknév: CHO Protein-Free Cell Line from Chinese hamster ovary cells, 00102307
• biológiai forrás: hamster ovary
• leírás: Chinese hamster ovary
• növekedési mód: Suspension
• kariotípus: Not specified
• morfológia: Suspension
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Chinese hamster ovary

Sejtvonal leírása

A subclone of the parental CHO line originated by Puck in 1957. The cells have an absolute requirement for L-proline. The cells have been adapted for growth in Sigma CHO Protein Free Medium (C5467). They have been cryopreserved in Sigma Cell Freezing Medium - Serum Free (C2639).

Táptalaj

Sigma CHO-protein free medium + 4mM Glutamine. Cells should be frozen in either Sigma Cell Freezing Medium - Serum Free (C2639) with 10% DMSO or Sigma CHO Protein Free Medium (C5467) with 7.5%-10% DMSO

Szubkultúra rutin

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 10⁵ cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen. Once established use a split ratio of 1:2 approximately every 4 to 5 days; 5% CO₂; 37°C.

Egyéb megjegyzések

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