Huh-7D 12 Cell Line human

1042712, human liver, Epithelial

• Szinonimák: Huh7 Cells, JTC-39 Cells, Japanese Tissue Culture-39 Cells
• UNSPSC kód: 41106514

Tulajdonságok

• product name: Huh-7D 12 Cell Line human, 01042712
• biológiai forrás: human liver
• növekedési mód: Adherent
• kariotípus: That of original Huh-7
• morfológia: Epithelial
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• vonatkozó betegség(ek): cancer
• tárolási hőmérséklet: −196°C

Leírás

Általános leírás

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised. Huh-7D is a derivative of Huh-7 cells, which favor hepatitis C virus replication. Huh-7 cells are superior hepatic cell models compared to HepG2 cells for apolipoprotein based metabolic studies.

Sejtvonal eredete

Human hepatocellular carcinoma

Sejtvonal leírása

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised.

Alkalmazás

Study of intracellular traffic of delta virus antigens and RNA, viral RNA replication and early virus assembly events, and interaction between HDV and HBV.

DNS profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16,18

Táptalaj

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37 C. Media change twice a week

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