Ugrás a tartalomra
Merck

C7926

Sigma-Aldrich

Collagenase from Clostridium histolyticum

Sigma Blend Type F, ≥2.0 FALGPA units/mg solid

Szinonimák:

Clostridiopeptidase A

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

CAS-szám:
Enzyme Commission szám:
EC-szám:
MDL-szám:
UNSPSC kód:
12352204
NACRES:
NA.54

biológiai forrás

Clostridium histolyticum

Minőségi szint

form

powder

caseinase activity

≤10 units/mg solid

specifikus aktivitás

≥2.0 FALGPA units/mg solid

molekulatömeg

68-130 kDa

tárolási hőmérséklet

−20°C

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Alkalmazás

Collagenase from Clostridium histolyticum has been used in determining the degradation rate of collagen biomaterials. It may also be used to determine the enzymatic degradation and enzymatic resistance by the liberated L-leucine measurements.
Collagenase from Clostridium histolyticum, or Clostridiopeptidase A, has been used in a study to assess contact dermatitis with clostridiopeptidase A contained in Noruxol ointment. Clostridiopeptidase A has also been used in a study to investigate the early and successful enzymatıc debridement via collagenase application to pinna in a preterm neonate.
The enzyme has been used in the isolation of mast cells from adult mice. This study generated a large number of connective tissue-type mast cells by the culture of murine fetal skin cells. The product has also been used in the in vitro biodegradation study of Bombyx mori silk fibroin fibers and films, by exposing them to the enzyme for varied time periods.

Biokémiai/fiziológiai hatások

Effective release of cells from tissue requires the action of collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA)4; β-mercaptoethanol; glutathione, reduced; thioglycolic acid, sodium; and 2,2′-dipyridyl; 8-hydroxyquinoline are known to inhibit the enzyme activity.
Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

Egység definíció

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

Piktogramok

Health hazardExclamation mark

Figyelmeztetés

Danger

Figyelmeztető mondatok

Veszélyességi osztályok

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Célzott szervek

Respiratory system

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Biodegradation of Bombyx mori silk fibroin fibers and films.
Arai T, et al.
Journal of Applied Polymer Science, 91(4), 2383-2390 (2004)
Nobuo Yamada et al.
The Journal of investigative dermatology, 121(6), 1425-1432 (2003-12-17)
We describe a novel culture system for generating large numbers of murine skin-associated mast cells and distinguish their characteristics from bone marrow-derived cultured mast cells. Culture of day 16 fetal skin single cell suspensions in the presence of interleukin-3 and
Properties of collagen and hyaluronic acid composite materials and their modification by chemical crosslinking
Rehakova M, et al.
Journal of Biomedical Materials Research, 30(3), 369-372 (1996)
K Vizárová et al.
Biomaterials, 16(16), 1217-1221 (1995-11-01)
Two kinds of layered atelocollagen materials cross-linked with hexamethylene diisocyanate (HMDIC), starch dialdehyde and glyoxal were enzymatically treated by bacterial collagenase. Evaluating collagenase digestion assay for these material showed progressive differences, particularly in the group of samples cross-linked with HMDIC.
Brittney R Garner et al.
The Journal of pharmacology and experimental therapeutics, 376(1), 40-50 (2020-10-27)
Pharmacological openers of ATP-sensitive potassium (KATP) channels are effective antihypertensive agents, but off-target effects, including severe peripheral edema, limit their clinical usefulness. It is presumed that the arterial dilation induced by KATP channel openers (KCOs) increases capillary pressure to promote

Protocols

To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

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