C3868

Anti-Calsequestrin-2 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Szinonimák: Anti-CASQ2, Anti-fast-twitch, cardiac muscle Calsequestrin
• UNSPSC kód: 12352203
• NACRES: NA.44

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 200
• konjugátum: unconjugated
• antitest forma: affinity isolated antibody
• antitest terméktípus: primary antibodies
• klón: polyclonal
• form: buffered aqueous solution
• molekulatömeg: antigen ~55 kDa
• faj reaktivitás: rat, human
• faj reaktivitás (homológia által előrejelzett): mouse
• fejlettebb validálás: recombinant expression; Learn more about Antibody Enhanced Validation
• koncentráció: ~1.0 mg/mL
• technika/technikák: western blot: 0.5-1.0 μg/mL using HEK-293T cells expressing human calsequestrin-2; western blot: 0.5-1.0 μg/mL using extract of rat cardiac muscle (S1 fraction)
• UniProt elérési szám: O14958
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... CASQ2(845) ; mouse ... Casq2(12373) ; rat ... Casq2(29209)

Leírás

Általános leírás

Calsequestrin is a major Ca²⁺ binding protein in cardiac and skeletal muscle.

Calsequestrin-2 (55 kDa) is found in both sarcoplasmic reticulum′s (SR) terminal cisternae luminal space of both cardiac and in slow skeletal muscle cells. It is made of three domains each with negatively charged thioredoxin-like folds with a β-sheet surrounded by four α-helices.

Immunogen

synthetic peptide corresponding to amino acids 57-74 of rat calsequestrin-2, conjugated to KLH. This sequence is highly conserved in human and rat calsequestrin-2 (single amino acid substitution). This sequence is not found in calsequestrin-1.

Alkalmazás

Anti-Calsequestrin-2 antibody has been used in immunoblotting, western blotting.

Anti-Calsequestrin-2 is suitable for western blot at a concentration of 0.5-1.0μg/mL using HEK-293T cells expressing human calsequestrin-2 and western blot at a concentration of 0.5-1.0μg/mL using extract of rat cardiac muscle (S1 fraction)

Biokémiai/fiziológiai hatások

Over expression of cardiac calsequestrin (CASQ2) is associated with depressed cardiovascular function and hypertrophy. Mutations in CASQ2 are thought to be associated with catecholaminergic polymorphic ventricular tachycardia (CPVT). It is a high-capacity, low-affinity Ca²⁺ binding glycoprotein, which functions as an internal Ca²⁺ store in the lumen of the sarcoplasmic reticulum (SR).

Fizikai forma

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)