B8438
Alcian Blue solution
1% in 3% acetic acid, pH 2.5
Szinonimák:
Alcian Blue 8XG solution
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12171500
NACRES:
NA.47
Javasolt termékek
Forma
liquid
Minőségi szint
koncentráció
1% in 3% acetic acid, pH 2.5
technika/technikák
microbe id | staining: suitable
szín
dark blue to very dark blue
pH-tartomány
2.4-2.6
Amax
0.45-0.60 (1:400 in H2O)
alkalmazás(ok)
diagnostic assay manufacturing
hematology
histology
tárolási hőmérséklet
room temp
Alkalmazás
Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.
Biokémiai/fiziológiai hatások
Alcian Blue is a cationic dye. It forms insoluble complexes with acidic glycosaminoglycans, thereby aiding in the quantitative determination of glycosaminoglycans. It is also used for the staining of bone and cartilage.
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
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Differential staining of acid glycosaminoglycans (mucopolysaccharides) by alcian blue in salt solutions.
J E Scott et al.
Histochemie. Histochemistry. Histochimie, 5(3), 221-233 (1965-10-01)
G F Pierce et al.
The American journal of pathology, 138(3), 629-646 (1991-03-01)
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SPECIFIC STAINING OF SULPHATE GROUPS WITH ALCIAN BLUE AT LOW PH.
R LEV et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 12, 309-309 (1964-04-01)
Hao Wu et al.
Experimental cell research, 361(2), 324-332 (2017-11-04)
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W Lin et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 45(8), 1157-1163 (1997-08-01)
The apparent intensity of hyaluronan (HA) staining in tissue sections can vary as a function of fixation techniques. We examined the histochemical distribution of HA in normal human skin using an HA-specific binding peptide derived from bovine nasal cartilage. The
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