A5268

Alcian Blue 8GX

powder

• Szinonimák: Alcian Blue, Ingrain Blue 1
• Tapasztalati képlet (Hill-képlet): C₅₆H₆₈Cl₄CuN₁₆S₄
• CAS-szám: 33864-99-2
• Molekulatömeg: 1298.86
• Colour Index szám: 74240
• EC-szám: 251-705-7
• MDL-szám: MFCD00010720
• UNSPSC kód: 12171500
• PubChem Substance ID: 24890962
• NACRES: NA.47

Tulajdonságok

• Forma: powder
• Minőségi szint: 100
• összetétel: Dye content, 45-65%
• technika/technikák: microbe id | staining: suitable
• szín: blue
• oldhatóság: H₂O: 1 mg/mL
• εmax: 425 at 615-630 nm in water
• alkalmazás(ok): diagnostic assay manufacturing; hematology; histology
• tárolási hőmérséklet: room temp
• SMILES string: [Cl-].[Cl-].[Cl-].[Cl-].CN(C)C(\SCc1ccc2c3nc(nc4n5[Cu]n6c(nc7nc(nc5c8cc(CS\C(N(C)C)=[N+](\C)C)ccc48)c9ccc(CS\C(N(C)C)=[N+](\C)C)cc79)c%10ccc(CS\C(N(C)C)=[N+](\C)C)cc%10c6n3)c2c1)=[N+](/C)C
• InChI: 1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-57-45(37)62-50-43-27-35(31-75-55(69(9)10)70(11)12)19-23-39(43)47(59-50)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(60-52)63-51-42-26-34(18-22-38(42)46(58-51)61-49)30-74-54(67(5)6)68(7)8;;;;;/h17-28H,29-32H2,1-16H3;4*1H;/q+2;;;;;+2/p-4
• Nemzetközi kémiai azonosító kulcs: CKLBXIYTBHXJEH-UHFFFAOYSA-J

Leírás

Általános leírás

Alcian Blue 8GX, also known as Ingrain Blue 1, is a basic copper phthalocyanine dye whose copper content imparts a blue color. It is tetravalent, cationic, and water-soluble in nature. It forms salt linkages with the acid groups of acid mucopolysaccharides.

Alkalmazás

• Alcian Blue 8GX has been used for the staining and precipitation of glycosaminoglycans and as a heteroglycan stain for neutral, sulfated, and phosphated mucopolysaccharides and glycosaminoglycans in tissues such as cartilage and extracellular matrices.
• It has been employed in the in vivo visualization of intravascular Bonghan ducts and corpuscles in murine models.
• It has been used to develop a novel histochemical staining method to assess multiple respiratory parameters in various species.

Tulajdonságok és előnyök

Desired staining patterns can be obtained by manipulating pH and salt concentrations.

Elv

The Alcian blue staining is easily combined with the Periodic acid-Schiff (PAS) reaction. In the tissue, acid mucosubstances are stained selectively and nuclear fast red is commonly used as counterstain. In combination with the PAS reaction, non-substituted polysaccharides, neutral mucopolysaccharides, muco- and glycoproteins, and glycol- and phospholipids can be visualized in addition. An Acetic acid-Alcian blue solution is used for the staining, whereby carboxylated and sulfatic proteoglycans are selectively stained when a pH of 2.5 is applied. The differentiation between carboxyl and sulfate groups is only possible at pH 1.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)