A9917
Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Szinonimák:
Goat Anti-Mouse IgG (Fab specific)–HRP
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
Javasolt termékek
biológiai forrás
goat
Minőségi szint
konjugátum
peroxidase conjugate
antitest forma
affinity isolated antibody
antitest terméktípus
secondary antibodies
klón
polyclonal
form
buffered aqueous solution
faj reaktivitás
mouse
technika/technikák
direct ELISA: 1:60,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150
western blot: 1:80,000-1:160,000
kiszállítva
dry ice
tárolási hőmérséklet
−20°C
célzott transzláció utáni módosítás
unmodified
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Általános leírás
Immunoglobulin G (IgG) is a glycoprotein antibody. IgG belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3.
Egyediség
Specificity of the Anti-Mouse IgG (Fab Specific)- Peroxidase is determined by immunoelectrophoresis (IEP), prior to conjugation. By IEP, single precipitation arcs are observed against normal mouse serum, mouse IgG and the Fab fragment of mouse IgG, while no reaction is observed against the Fc fragment of mouse IgG or human IgG. Binds all mouse IgGs.
Immunogen
Purified mouse IgG Fab fragment
Alkalmazás
Murine IgG levels were quantitated in brain abscess homogenates by ELISA using HRP conjugated goat anti-mouse Fab specific antibody as the detection antibody.
Anti-Mouse IgG (Fab specific)-Peroxidase antibody has been used in immunofluorescence, ELISA (enzyme-linked immunosorbent assay) and immunoblotting.
Goat Anti-Mouse IgG (Fab specific)-Peroxidase antibody can be used for western blotting assays. The product can also be used for direct ELISA (1:60,000) and IHC (1:150) applications.
Goat Anti-Mouse IgG (Fab specific)-Peroxidase antibody can be used for western blotting assays. The product can also be used for direct ELISA (1:60,000) and IHC (1:150) applications.
Bovine CVEC and human epidermoid carcinoma A431 cell lysates were analyzed by western blot using HRP-conjugated goat anti-mouse IgG Fab specific as the secondary antibody at a 1:3000 dilution.
Biokémiai/fiziológiai hatások
Immunoglobulin G (IgG) regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG1 regulates complement fixation, opsonization and antibody dependent cell mediated cytotoxicity in mice. IgG participates in hypersensitivity type II and type III.
Egyéb megjegyzések
Antibody adsorbed with human IgG.
Fizikai forma
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.
Elkészítési megjegyzés
Adsorbed to reduce background staining with human samples.
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Tárolás és stabilitás
Store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Figyelmeztetés
Warning
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Skin Sens. 1
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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