A9044

Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Szinonimák: Anti Mouse Antibody, Anti Mouse Antibody - Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in rabbit, Anti Mouse Hrp, Anti Mouse Hrp Sigma, Anti Mouse Igg, Sigma Anti Mouse Hrp
• MDL-szám: MFCD00162644
• UNSPSC kód: 12352203
• NACRES: NA.46

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 300
• konjugátum: peroxidase conjugate
• antitest forma: IgG fraction of antiserum
• antitest terméktípus: secondary antibodies
• klón: polyclonal
• form: buffered aqueous solution
• technika/technikák: direct ELISA: 1:40,000 using using 5 μg/ml of mouse IgG for coating and OPD substrate; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200; western blot (chemiluminescent): 1:80,000-160,000
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified

Leírás

Általános leírás

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids.
IgG antibody is a glycoprotein, composed of two polypeptide chains (light and heavy), each possessing variable and constant domains. The variable region of IgG antibody is specific to antigens and is highly conserved.
Anti-Mouse IgG (whole molecule)-Peroxidase antibody is specific for all mouse IgG subclasses. IgG is purified from mouse serum by fractionation and ion exchange chromatography. The purified IgG is then conjugated to peroxidase by protein cross-linking with 0.2% glutaraldehyde.

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein and a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Egyediség

Anti-Mouse IgG (whole molecule)-Peroxidase antibody is specific for all mouse IgG subclasses.

Immunogen

Purified mouse IgG

Alkalmazás

Anti-Mouse IgG (whole molecule)–Peroxidase antibody has been used in western blotting , immunohistochemistry and protein pin array assay.

Anti-Mouse IgG (whole molecule)–Peroxidase antibody may be used in direct ELISA at a working antibody dilution of 1:40,000. For immunoblotting, a dilution of 1:80,000 to 1:160,000 may be used. A working antibody dilution of 1:200 is recommended for immunohistochemistry of formalin-fixed, paraffin-embedded human tonsil or human appendix sections. Mouse IgG-peroxidase antibody was used at a dilution of 1:20 for immunohistochemistry of mice muscle tissue sections; a dilution of 1:500 was used for South-western blotting in the same study. For immunoblotting of human skeletal muscle tissue extracts, a dilution of 1:5000 was used.

Epidermis protein extracts were generated from human mammary surgery specimens and subjected to western blot using HRP-conjugated rabbit anti-mouse IgG as the secondary at a 1:80000 dilution in TBSt/5% milk.

Biokémiai/fiziológiai hatások

IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgG antibody has its function similar to IgM antibody in complement system activation.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Elkészítési megjegyzés

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Tárolás és stabilitás

Store at -20 °C for long term. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots -20 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Piktogramok: GHS08
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H317,H334
• Óvintézkedésre vonatkozó mondatok: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Veszélyességi osztályok: Resp. Sens. 1 - Skin Sens. 1
• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable