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Fontos dokumentumok
A5691
Anti-Actin, α-Smooth Muscle - Alkaline Phosphatase antibody, Mouse monoclonal
clone 1A4, purified from hybridoma cell culture
Szinonimák:
Monoclonal Anti-Actin, α-Smooth Muscle, Monoclonal Anti-Actin, α-Smooth Muscle - Alkaline Phosphatase antibody produced in mouse, SMA
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
UNSPSC kód:
12352203
NACRES:
NA.41
konjugátum:
alkaline phosphatase conjugate
application:
ELISA
IHC (p)
WB
IHC (p)
WB
klón:
1A4, monoclonal
faj reaktivitás:
human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
citations:
77
technika/technikák:
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human tonsil or appendix sections
western blot: 1:100 using chicken gizzard extract/ Mouse heart extract
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human tonsil or appendix sections
western blot: 1:100 using chicken gizzard extract/ Mouse heart extract
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
konjugátum
alkaline phosphatase conjugate
antitest forma
purified from hybridoma cell culture
antitest terméktípus
primary antibodies
klón
1A4, monoclonal
Forma
buffered aqueous glycerol solution
molekulatömeg
antigen ~42 kDa
faj reaktivitás
human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
technika/technikák
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human tonsil or appendix sections
western blot: 1:100 using chicken gizzard extract/ Mouse heart extract
izotípus
IgG2a
UniProt elérési szám
kiszállítva
wet ice
tárolási hőmérséklet
2-8°C
célzott transzláció utáni módosítás
unmodified
Géninformáció
mouse ... Acta2(11475)
rat ... Acta2(81633)
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Általános leírás
Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two γ actins (γ smooth muscle and γ non-muscle).
The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.
Immunogén
N-terminal synthetic decapeptide of α-smooth muscle actin.
Alkalmazás
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Immunohistochemistry (1 paper)
Immunocytochemistry was performed on kertocytes fixed in 1% PFA and incubated with mouse monoclonal anti-smooth muscle actin (14A) at a 1:100 dilution.
Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.
Mouse monoclonal anti-actin, α−smooth muscle-alkaline phosphatase antibody has been used for immunohistochemical application in mouse and human tissues.
Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
Fizikai forma
Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide as a preservative.
Egyéb megjegyzések
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 2
Egyéni védőeszköz
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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