C6198

Anti-Actin, α-Smooth Muscle - Cy3^™ antibody, Mouse monoclonal

clone 1A4, purified from hybridoma cell culture

• Szinonimák: Monoclonal Anti-Actin, α-Smooth Muscle, Alpha-actin 2 Cy3, alpha-smooth muscle actin - Cy3^™ antibody, SMA
• UNSPSC kód: 12352203
• NACRES: NA.43
• konjugátum: CY3 conjugate
• application: IHC (p)
• klón: 1A4, monoclonal
• faj reaktivitás: human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
• citations: 896
• technika/technikák: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tonsil or appendix

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 200
• konjugátum: CY3 conjugate
• antitest forma: purified immunoglobulin
• antitest terméktípus: primary antibodies
• klón: 1A4, monoclonal
• Forma: buffered aqueous solution
• molekulatömeg: antigen ~42 kDa
• faj reaktivitás: human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
• tárolási körülmény: protect from light
• technika/technikák: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tonsil or appendix
• izotípus: IgG2a
• UniProt elérési szám: P62736
• alkalmazás(ok): research pathology
• kiszállítva: wet ice
• tárolási hőmérséklet: 2-8°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... ACTA2(59) ; mouse ... Acta2(11475) ; rat ... Acta2(81633)

Leírás

Általános leírás

Monoclonal Anti-Actin, α-Smooth Muscle (mouse IgG2a isotype) is derived from the 1A4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes of immunized BALB/c mice.

The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.

Immunogén

N-terminal synthetic decapeptide of α-smooth muscle actin.

Alkalmazás

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (2 papers)
Immunocytochemistry (3 papers)
Immunofluorescence (13 papers)
Immunohistochemistry (27 papers)
Western Blotting (2 papers)

Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.

Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.

Biokémiai/fiziológiai hatások

Smooth muscle actin is transiently coexpressed with sarcomeric α-actin during myogenesis in chicken and rat embryos. It has been found in the ventricular conducting tract of adult mammalian heart. It is expressed in leiomyomas, leiomyosarcomas and leiomyoblastomas, as well as in a proportion of rhabdomyosarcomas.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide, as a preservative.

Egyéb megjegyzések

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Jogi információk

Cy is distributed under license from Amersham Biosciences Limited.

Cy3 is a trademark of Cytiva

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable