83264

HEPES solution

1 M in H₂O, liquid

• Szinonimák: HEPES solution, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• CAS-szám: 7365-45-9
• MDL-szám: MFCD00006158
• UNSPSC kód: 12161700
• eCl@ss: 32129211
• PubChem Substance ID: 57652951
• NACRES: NA.25

Tulajdonságok

• Terméknév: HEPES buffer solution, 1 M in H₂O
• Forma: liquid
• Minőségi szint: 100
• koncentráció: 1 M in H₂O; 238 g/L HEPES in H₂O
• szennyeződések: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pH: 5.5±0.5
• hasznos pH-tartomány: 6.8-8.2
• sűrűség: 1.07 g/mL at 20 °C
• alkalmazás(ok): diagnostic assay manufacturing
• SMILES string: OCCN1CCN(CCS(=O)(O)=O)CC1
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• Nemzetközi kémiai azonosító kulcs: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Leírás

Általános leírás

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Alkalmazás

HEPES has been used:

• As a component of SAPI medium used for the culturing of S. Typhymurium inoculum
• To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI
• As a component of dissection buffer for culturing rat cortical neurons
• Along with MgCl₂, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses
• As a component of Danieu′s aquarium water used in agarose gel preparation

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)