54457

HEPES

≥99.5% (titration), powder, for molecular biology, BioUltra

• Szinonimák: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Tapasztalati képlet (Hill-képlet): C₈H₁₈N₂O₄S
• CAS-szám: 7365-45-9
• Molekulatömeg: 238.30
• Beilstein: 883043
• EC-szám: 230-907-9
• MDL-szám: MFCD00006158
• UNSPSC kód: 12161700
• PubChem Substance ID: 57651473
• NACRES: NA.25

Tulajdonságok

• product name: HEPES, BioUltra, for molecular biology, ≥99.5% (T)
• grade: for molecular biology
• Minőségi szint: 200
• termékcsalád: BioUltra
• Teszt: ≥99.5% (T)
• form: powder
• szennyeződések: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• izzítási maradék: ≤0.1% (as SO₄)
• veszteség: ≤0.5% loss on drying, 110 °C
• pH: 5.0-6.5 (25 °C, 1 M in H₂O)
• hasznos pH-tartomány: 6.8-8.2
• pKa (25 °C): 7.5
• oldhatóság: H₂O: 1 M at 20 °C, clear, colorless
• anion nyomok: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• kation nyomok: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 1 M in H₂O
• UV-elnyelés: λ: 260 nm A_max: 0.045; λ: 280 nm A_max: 0.035
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• Nemzetközi kémiai azonosító kulcs: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Leírás

Általános leírás

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Alkalmazás

HEPES has been used as a component of:

• DMEM (Dulbecco′s modified Eagle′s medium) used for the cultivation of CHO-GPR40?cells
• RNA buffer (for gel-filtration chromatography) and transcription buffer (for transcription reaction)
• Recapture solution to re-capture RNA exosomes from the glycerol gradient
• ExoI and ExoII extraction solution as well as light and heavy gradient solution for gradient centrifugation
• 2X HBS buffer that is used in transfection process for the production of recombinant lentivectors

Egyéb megjegyzések

Easily compare specifications for HEPES products with the HEPES specification table.

3′-Terminal labeling of RNA with T₄ RNA ligase; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer.

Biztonsági adatok

• Tárolási osztály kódja: 13 - Non Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)