H7006

HEPES sodium salt

≥99.0% (titration)

• Szinonimák: Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
• Tapasztalati képlet (Hill-képlet): C₈H₁₇N₂NaO₄S
• CAS-szám: 75277-39-3
• Molekulatömeg: 260.29
• EC-szám: 278-169-7
• MDL-szám: MFCD00036463
• UNSPSC kód: 12161700
• PubChem Substance ID: 24895780
• NACRES: NA.25

Tulajdonságok

• Minőségi szint: 400
• Teszt: ≥99.0% (titration)
• Forma: powder
• tárolási körülmény: dry at room temperature
• technika/technikák: HPLC: suitable
• szín: white
• pH: 10-12 (1 M in water)
• hasznos pH-tartomány: 6.8-8.2
• pKa (25 °C): 7.5
• oldhatóság: water: 0.1 M, clear, colorless
• alkalmasság: suitable for mass spectrometry
• alkalmazás(ok): diagnostic assay manufacturing; general analytical (; ); life science and biopharma; metabolomics
• tárolási hőmérséklet: room temp
• SMILES string: [Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
• InChI: 1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
• Nemzetközi kémiai azonosító kulcs: RDZTWEVXRGYCFV-UHFFFAOYSA-M

Leírás

Általános leírás

HEPES salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, is a zwitterionic biological buffer that is widely used in biological and biochemical research. It has a buffering range of pH 6.8 to 8.2, making it ideal for a variety of research applications. HEPES is also a popular choice for buffering cell culture media, offering advantages such as lower cell toxicity in comparison to alternatives like Tris and phosphate.

Widely utilized in cell biology and biochemical research, HEPES proves its versatility in protein purification and extraction protocols, owing to its stability across a broad pH range and minimal metal ion binding. Additionally, HEPES proves valuable in various biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging, making it a versatile tool in diverse research applications.

Alkalmazás

HEPES buffer finds application in:

• Most studies with metal ions
• Substitute for Tris and phosphate in studies with metal ions
• Grinding buffer in plant studies
• Running buffer in gel electrophoresis
• Buffer in electroporation studies
• Bradford or bicinchoninic acid (BCA) assays
• Extraction buffer to prevent damage of proteins in red blood cells

It has been used:

• as a buffer component in the preparation of a low sucrose buffer
• as a buffer component in a modified Hanks′ solution, a pseudo-physiological solutions

Tulajdonságok és előnyök

• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Highly soluble in water
• Negligible metal ion binding
• Less toxic to cells than other buffers such as Tris and phosphate
• Stable in a wide pH range

Fizikai forma

Lyophilized, spray dried, or crystallized material.

Egyéb megjegyzések

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Biztonsági adatok

• Tárolási osztály kódja: 13 - Non Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)