PPB015

Tris-Glycine Buffer

pH 8.3, pHast Pack^™, powder

• Szinonimák: 1X TG buffer, TG, TG Buffer, Tris Glycine running buffer, Tris Glycine transfer buffer, Tris glycine
• UNSPSC kód: 41105319
• NACRES: NA.25

Tulajdonságok

• termékcsalád: pHast Pack^™
• Minőségi szint: 100
• form: powder
• tárolási körülmény: (Tightly closed. Dry)
• szennyeződések: ≤5 ppm Heavy Metals (lead)
• szín: white to off-white
• pH: 8.3
• oldhatóság: soluble, clear, colorless to faintly yellow
• kation nyomok: Fe: <10 ppm
• idegen aktivitás: DNase, none detected; Endonuclease ; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• tárolási hőmérséklet: room temp

Leírás

Általános leírás

Tris-Glycine buffer is primarily used as a running buffer and to prepare transfer buffer in western blot analysis with the addition of methanol. This buffer can separate proteins by their size. It has a pH of 8.3.

Alkalmazás

Suitable for use as:

• Transfer buffer component in western blot
• Running buffer in native gel electrophoresis for protein separation
• Running buffer in gradient polyacrylamide gel electrophoresis (PAGE)

Tulajdonságok és előnyök

• Ready-to-use buffer
• No measuring and pH adjusting needed
• Saves time and minimizes the effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Kiszerelés

Foil pouches

Feloldás

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1x solution containing 25 mM Tris, 192 mM Glycine, pH 8.3 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

Tárolás és stabilitás

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Egyéb megjegyzések

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Jogi információk

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1