T7777

Tris-Glycine-SDS Buffer 10× Concentrate

• Szinonimák: TRIS glycine SDS buffer solution
• UNSPSC kód: 41105319
• eCl@ss: 32129211
• NACRES: NA.25

Tulajdonságok

• sterilitás: 0.2 μm filtered
• Minőségi szint: 200
• form: liquid
• alkalmazás(ok): diagnostic assay manufacturing
• tárolási hőmérséklet: 2-8°C

Leírás

Általános leírás

Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both anode and cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.

Alkalmazás

Tris-glycine-SDS buffer 10× concentrate has been used as a running buffer in sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE). It is also used to block membranes with 5% non-fat dry milk for western blotting. TGS is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.

Feloldás

Dilution of the 10× TGS buffer produces a 1× running buffer containing 25 mM Tris, 192 mM glycine and 0.1% SDS, pH approx. 8.6.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type ABEK (EN14387) respirator filter