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114391

Sigma-Aldrich

Bromophenol Blue

ACS reagent

Szinonimák:

3′,3″,5′,5″-Tetrabromophenolsulfonphthalein, Bromphenol Blue Sultone Form

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About This Item

Tapasztalati képlet (Hill-képlet):
C19H10Br4O5S
CAS-szám:
115-39-9
Molekulatömeg:
669.96
Beilstein:
61698
EC-szám:
204-086-2
MDL-szám:
MFCD00005875
UNSPSC kód:
12171500
PubChem Substance ID:
24847169
NACRES:
NA.47

grade

ACS reagent

Minőségi szint

200

form

powder

technika/technikák

titration: suitable

visual transition interval

3.0-4.6, yellow to blue

mp

273 °C (lit.)

oldhatóság

ethanol: 0.1%

alkalmazás(ok)

diagnostic assay manufacturing
hematology
histology

tárolási hőmérséklet

room temp

SMILES string

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4

InChI

1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H

Nemzetközi kémiai azonosító kulcs

UDSAIICHUKSCKT-UHFFFAOYSA-N

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Általános leírás

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein.Bromophenol Blue is a pH indicator and a dye appearing as a strong blue color. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Alkalmazás

Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. It has also been known for its use as a vital stain to probe the blood-brain barrier.

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, type N95 (US)

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
SHBR7468
BCCM1010
SHBR1340
BCCJ9036
BCCK2682

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COA keresése

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